The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
In this work, we present a fluorescent on-chip imaging system utilizing a custom-made dye-doped-resin type filter. The filter used in the system was characterized to determine its transmission properties. Unique steep cut-off property of the filter allows to align emission wavelength of the fluorophores to provide better signal-to-noise ratio (SNR). We precisely showed that fluorescent micro-objects...
In this paper, we report on the design, fabrication and characterization of a low cost, portable detection system able to quantify, in a rapid and reliable way, the contamination level of Ochratoxin A (OTA) in red wine. The operating principle is the real-time monitoring of the natural fluorescence of OTA molecules during a chromatographic run on a thin layer chromatographic plate. The fluorescence...
We have designed, fabricated, and tested a multi-spectral imager for near-infrared fluorescence image-guided surgery capable of simultaneously imaging both color and near-infrared fluorescence information with high sensitivity at 40 frames per second. The imaging sensor is composed of 1600 by 1200 imaging elements covered by pixelated spectral interference filters. The imaging sensor was successfully...
We have designed, fabricated, and tested a multi-spectral imager for near-infrared fluorescence image-guided surgery capable of simultaneously imaging both color and near-infrared fluorescence information with high sensitivity at 40 frames per second. The imaging sensor is composed of 1600 by 1200 imaging elements covered by pixelated spectral interference filters. The imaging sensor was successfully...
Integration and miniaturization of bio-molecular detection systems into electronic biosensors and lab-on-chip platforms is of great importance. One widely recognized application area for such devices is nucleic acid (DNA and RNA) detection, specifically, nucleic acid amplification testing (NAAT), which relies on enzymatic processes such as polymerase chain reaction (PCR) to increase the copy number...
Lateral flow immunoassay (LFIA) is widely utilized in a variety of point-of-care-test (POCT) applications. In this paper, we discuss the development of an ASIC based strip reader controller for LFIA. The control algorithm was proposed and implemented in Verilog HDL and fabricated into ASIC with 0.18 μm CMOS technology. Functional validation on both FPGA and ASIC implementations were presented. The...
The high per patient cost of quantitative, high-sensitivity molecular diagnostics is one of the key roadblocks limiting the transition of this technology from the clinical laboratory to point-of-care diagnostics in low-to-middle income countries (LMICs). As a solution, we present a low-cost system architecture for fluorescence-based point-of-care diagnostics using small volume patient sera samples...
A low cost and highly sensitive fluorescence detection system using smart phone cameras was developed for biological and biochemical detection experiments. The system was designed for standard 96-well plates, which are typically used in life-sciences laboratories. Traditional fluorescence detection is done using specialized optics, filters and ultra-sensitive detectors such as photo-multipliers, which...
A compact optical spectrometer approach is presented that integrates multiple linear variable filters (LVFs) onto a CMOS imaging sensor for measurement of liquid-phase fluorescence and absorption that provides a 3.77–6.08 nm spectral resolution over a 400–1050 nm wavelength range. For fluorescence detection, we demonstrate detection limits of 0.28 nM for Quantum Dot emitters and 32 ng/mL for near-infrared...
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free,...
Fluorescence immune-chromatography is a kind of immunoassay which is widely used in quantitative detection area. However, the background baseline drift with relatively large amplitude during test is a huge problem for the overall performance of the quantitative detection system, and the existing background baseline recognition and adjustment methods were based on online or offline data processing,...
A 110 × 64 pixel CMOS image sensor integrated with pixelated spectral interference filters is used for image guided surgery. The sensor operates with short exposure times for capturing visible light using blue, green, and red pixelated interference filters, and long exposure time for capturing near-infrared fluorescence with a co-located, pixelated NIR filter. The sensor was used to successfully detect...
A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array...
The paper presents an optoelectronic module for quantification of biomarkers based on fluorescence, intended to be used for detection of acute myocardial infarction (AMI). The basic principles of fluorescence microscopy have been used for designing this module, taking into account that the fluorophores used to mark the detection antibodies are quantum dots (QD) made of cadmium telluride (CdTe) with...
A Standoff detection scheme for buried landmines and concealed explosive charges based on genetically engineered bacterial biosensors is presented. Concentrations of 4 mg/L of 2,4-DNT on soil were detected from a distance of 50 meters.
We demonstrated two-photon excitation of fluorescent proteins by using visible light. The excitation scheme has been applied to simultaneous multicolor imaging of biological cells with improved spatial resolution.
The identification of phytoplankton is currently an important issue to prevent the aquatic environment. The growth of one or several phytoplankton species can lead to hyper eutrophication and causes lethal consequences on other organisms. In this paper, the selective recognition of invading species is investigated by automatic recognition algorithms of optical and fluorescence imaging. Firstly, morphological...
An image processing platform has been proposed aiming to develop expendable bio-sensing system with arrayed biosensor and embedded imager. For rapid and iterative prototyping in a variety of imagers, the platform is designed to be independent of the actual imager by giving us a unified interface to control the actual imager such as exposure, gain and acquisition timing. It also gives us various functions...
Affinity-based fluorescence sensing has been one of the key enabling technologies in biomolecular sensing, used for detection of proteins, DNAs, toxins, bacteria, etc, and remains one of the most sensitive, specific, robust, and widely used diagnostics methodology [1-4]. In absence of high-performance integrated optical filters, miniaturization of a fluorescence sensing system in CMOS has relied on...
This work presents the design, fabrication and characterization of a system based on thin film technology for the selective detection of the natural fluorescence of Ochratoxin A. To this aim, the system optically couples an amorphous silicon photosensor with a long pass multi-dielectric filter, deposited on glass substrates. In particular, the filter rejects the wavelengths coming from the excitation...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.