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Many computation kernels that analyze large data streams can be accelerated by converting their recurrences to parallel systolic arrays. Application domains such as bioinformatics seek to minimize the total time to analyze a large set of discrete small inputs. While traditional methods for array synthesis produce a single most efficient array design, modern computational platforms support fast runtime...
The specific segment in 3' terminal region of strawberry mild yellow edge virus (SMYEV) genome was amplified by 3' rapid amplification of cDNA ends (RACE). Ten Chinese isolates were sequenced. Sequence analysis of coat protein gene showed nucleotide and deduced amino acid identities ranged from 76.8 to 100% and 86.4 to 100%, respectively. Phylogenetic analysis showed that all isolates of SMYEV fell...
In this paper, we successfully predicted 16 microRNAs in 12 scaffold sequences of Strongylocentrotus.purpuratus genome by using a newly developed BNMir-P program which based on bayesian network, and further validity it in second round prediction by a different miPred software which integrated structure and sequence characteristic on known microRNA and a novel machine-learned algorithm termed random...
Oligochitosan is a potent plant defense elicitor, but how oligochitosan induces the resistance of plants to pathogens is still unclear. Intend to explore the mechanism of oligochitosan inducing defense, we cloned a new cDNA coding for S-phase kinase-associated protein 1(SKP1), designated BnSKPl (Genebank: DQ206627), from oilseed (Brassica Napus L) based on our former microarray data by RACE and bioinformatics...
Total RNA from Goose liver of 16 weeks old was isolated. The 365 bp cDNA segment was amplified by the reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis showed that there is a high similarity between the fragment of cDNA and Sterol regulatory element binding protein-2 (SREBP-2) gene. This segment contains a bHLH motif, basic region and leucine zipper domain. Meanwhile, the...
It is a classical result of Stein and Waterman that the asymptotic number S(n) of RNA secondary structures is 1.104366 ldr n-3/2 ldr 2.618034n, where the combinatorial model of RNA concerns a length n homopolymer, such that any base can pair with any other base, subject to the usual convention that hairpin loops must contain at least thetas = 1 unpaired bases. The result of Stein and Waterman is proved...
This paper describes a diagnostics strategy involving sample preparation, isothermal Nucleic Acid Sequence Based Amplification (NASBA) of bacterial tmRNA, fluorescent labeling, and microarray hybridisation to detect and identify Streptococcus pneumoniae at a concentration of less than one colony forming unit (CFU) per ml of culture. The tmRNA transcript of the bacterial ssrA gene exhibits several...
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