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Nowadays non-cellulosic, β-glucans from the different source are intensively investigated due to the fact that these biological polymers are recognized as potent immunological stimulators for the mammal's immune system. Dendritic cells are the most important antigen presenting cells for activating naive T cells. The research goals of the recent investigation were to determine how the different molecular...
An expanding body of work suggests that the tumor stroma plays an integral role in the initiation and development of epithelial cancers. Within the stroma, cancer-associated fibroblasts (CAFs) are involved in several functions that form key aspects of tumor progression, including immune suppression, metabolism, and invasion and metastasis. Despite the demonstrated importance of CAFs within the tumor...
Alpha-fetoprotein (AFP) is an oncofetal antigen during hepatocellular carcinoma (HCC) development which could lead to weak reproducible antitumor immunity, and may act as a target for cancer therapy. Therefore, it is imperative to enhance its immunogenicity and develop therapeutic vaccines to eliminate AFP-expressing tumors. In this study, by way of glutaraldehyde cross-linking, we constructed a potential...
Observe the influence of solasodine hydrochloride (SBHL) on mouse S180's tumor model's acquired multi-drug resistance by measuring the tumor chemo sensitivity, tumor cell apoptosis and gene expression level of P-glycoprotein(P-gp), Lung Cancer Resistance Protein(LRP) and Topoisomerase II. Methods Establish the Multi-drug Resistance (MDR) mouse S180 tumor model by low dose chemotherapy. Treat the mouse...
Okadaic acid (OA), a key diarrheic shellfish poisoning (DSP) toxin will possibly arouses DSP symptoms by consuming the contaminated shellfish. The DSP toxins are stable at high temperatures, and long-term DSP toxicity is carcinogenic. Therefore a fast and reliable analytical method for the detection of OA and analogues in shellfish is worth developing. In this paper, a direct competitive ELISA (dcELISA)...
Legionella pneumophila is an intracellular bacteria, and its successful parasitism in host cells involves two reciprocal phases: transmission and intracellular replication. In this study, we try to construct expression vectors of pcDNA3.1 (+)-lvgA/ctxB-EGFP fused gene and to investigate its transient expressions in NIH3T3 cells. Then preliminary analysis the immunogenicity of lvgA/ctxB fusion gene...
The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted...
In this study, BCGHSP70-B16TCL was prepared by directly mixing the BCG derived heat shock protein 70(BCGHSP70) with tumor cell lysate of B16 melanoma cells (B16TCL) in vitro and then administered to the subcutaneous B16 melanoma-bearing mice in the prophylactic immunization strategy to observe its anti-tumor effects. Data showed that prophylactic immunization with BCGHSP70-B16TCL could significantly...
In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS-PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein...
For raising the antigenicity of Mycobacterium bovis single antigen, fusion protein of two genes was acquired. The DNA fragments of mpb83 and ag85b were fused by splicing by overlapping extension (SOE) polymerase chain reaction(PCR), and the fusion gene mpb83-ag85b were cloned into pMD18-T vector, then we got the recombinant plasmid pMD-83-85b. pMD-83-85b and pET28a(+) were digested by BamH I and EcoR...
In order to study the biological characteristics of swine chemokine and the effect of Interferon-inducible Protein (IP-10) on the immunocompetence of vaccine, swine IP-10 gene was amplified from Spleen by RT-PCR, and the fragment was cloned into the eukaryotic expression plasmid to construct recombinant plasmid pcDNA3.1-IP-10. Then the plasmid pcDNA3.1-IP-10 was transfected into HEK293T cell. The...
This work addresses the development of artificial cellular niches for studying the mechanisms that affect stem cell pluripotency, and aims at contributing to a better understanding of the cellular and molecular events that regulate stem cell functions. Embryonic stem (ES) cells were used as model systems to establish in vitro artificial microenvironments that better mimic the extracellular matrix...
Ginger (Zingiber officinale) is used as a cooking spice in the diet in many parts of the world. Recent research has found ginger to have various pharmacological properties due to a variety of active constituents, including shogaols and gingerols. The aim of the present study was to assess the effect of alcohol extract of Zingiber officinale on the fractions of serum protein in mice. For this purpose,...
We studied the Fas/anti-Fas system to induce killing of Fas-expressing immunocytes with resultant immunosuppression. W7TM-1 tumour cells, a rat T-cell line, were inoculated subcutaneously in BALB/c mice and tumour growth was monitored in untreated mice and in mice treated with RMF2. Prior to treatment with RMF2, we examined the expression of Fas in isolated splenocytes and in tumour infiltrating lymphocytes...
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with...
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