The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
For raising the antigenicity of Mycobacterium bovis single antigen, fusion protein of two genes was acquired. The DNA fragments of mpb83 and ag85b were fused by splicing by overlapping extension (SOE) polymerase chain reaction(PCR), and the fusion gene mpb83-ag85b were cloned into pMD18-T vector, then we got the recombinant plasmid pMD-83-85b. pMD-83-85b and pET28a(+) were digested by BamH I and EcoR...
In order to study the biological characteristics of swine chemokine and the effect of Interferon-inducible Protein (IP-10) on the immunocompetence of vaccine, swine IP-10 gene was amplified from Spleen by RT-PCR, and the fragment was cloned into the eukaryotic expression plasmid to construct recombinant plasmid pcDNA3.1-IP-10. Then the plasmid pcDNA3.1-IP-10 was transfected into HEK293T cell. The...
A rapid method for detection diarrhetic shellfish poisoning (DSP), a marine biotoxin, were developed. Monoclonal antibody against OA (okadaic acid) was prepared by using the technique of cell fusion. This antibody was applied in development the idc-ELISA (indirect competitive enzyme-linked immunosorbent assay) for detection of OA. Using OA-KLH as the immunogen to immunize BALB/c mice, splenocytes...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.