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Salmonella pullorum was induced using liquid lysogeny broth medium at 4°C into viable but non-culturable (VBNC) state. The activated VBNC genes were screened by mRNA differential display. VBNC bacteria transformed to the spherical-rods or spherical shape and aggregated. Sequence analysis showed two VBNC differential fragments respectively belonged to partial sequences of ATP-dependent RNA unwindase...
A simple and rapid micellar electrokinetic capillary chromatography (MEKC) method has been developed for the simultaneous determination of six lignans, schizandrin, Gomisin A, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C, in fruits of S. chinensis and S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained,...
The present study was conducted to elucidate the immunomodulatory effect of a lectin from Musca domestic pupa (MPL) in vivo. The effects of MPL (200, 400 and 800 μg/kg body wt.) on immune organ weight, mitogen-induced proliferation of spleen lymphocyte, phagocytosis activity of macrophages, NK cell activity, and the mRNA expression of immune factor in spleen like interleukin (IL) and interferon (IFN)...
Establish a method of cultivation and purification of dendritic cells (DCs) from mouse bone marrow in vitro to provide experimental material and establish basis for the study of immunological tolerance. BALB/c (H-2d) mice spleen lymphocytes and bone marrow cells were extracted under sterile conditions and cultured in RPMI 1640 medium. DCs were induced by recombinant granulocyte macrophage colony stimulating...
To study the role of CD4+ CD25+ Treg cells in anti-graft rejection response and potential value in application of lung transplatation, we established mice trachea allograft transplant model and sort CD4+ CD25+ Treg cells from mice spleen lymphocytes by MACS. FCM assays were done to analyze the ratio of marker protein, Foxp3, expression in CD4+ CD25+ Treg cells. Alveolar cells were used as a non-specific...
To improve a capillary electrophoresis method used for the rapid and simultaneous separation and determination of rutin, chlorogenic acid and quercetin. The capillary column without coating (50μm×56cm, the used length: 47.5cm) was used, the solution of 12mM sodium tetraborate pentahydrate (pH 9.0) containing 15% methanol was taken as the running buffer and the temperature was 25 _. The linear relationship,...
To explore the association between dibutyl phthalate (DBP)-induced testicular function damage and endocrine disorders by using adult male rats as animal models. 40 healthy male SD rats were randomly divided into three exposure groups (DBP: 1000mg. kg−1. d−1, 500mg. kg−1. d−1, 50mg. kg−1. d−1) and one negative control group (corn oil by gavage: 10mL.kg.d-1), and were killed by cervical dislocation...
A quantitative determination of the total alkaloid in Corydalis Ochotensis Turcz was established by acid dye colorimetry. The effects of wavelength, pH of the phosphate buffer and the quantity of acid dye were studied. Two different dyes were compared according to the yield of berberine hydrochloride. The method with bromophenol blue dye was proved to be more accurate, more stable with good reproducibility...
The preliminary laboratory study found that Prunus Tomentosa Thumb Total Flavone (PTTTF) had certain protection function on skin tissues and its subsidiary organs. Combined with civilian application experience, the study adopted C57BL 6 mouse phalacrosis models, the models were randomly divided into 5 groups with 10 in each group, namely: saline group (1 time / day), 70% ethanol group (1 time / day),...
The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted...
Legionella pneumophila is an intracellular bacteria, and its successful parasitism in host cells involves two reciprocal phases: transmission and intracellular replication. In this study, we try to construct expression vectors of pcDNA3.1 (+)-lvgA/ctxB-EGFP fused gene and to investigate its transient expressions in NIH3T3 cells. Then preliminary analysis the immunogenicity of lvgA/ctxB fusion gene...
This paper was to design a cyclic RGD (Arg-Gly-Asp) peptide tumor inhibitor with high affinity to integrin αvβ3 receptor by molecular docking technique, determinate the characteristics of the cRGD dimer in vitro and prepare 99mTc-cRGD dimer tumor probe. cRGD molecule library was built and an optimal structure of cRGD peptide with the best score that was Cys-Arg-Gly-Asp-(D)Ser-Cys was screened out...
The paper reports an identification method of Semen Cuscutae. The ultramicofingerprint features of Semen Cuscutae is probed with scanning electron microscope and other two different methods, which researched on standard sample and random samples. There is an obvious difference between Semen Cuscutae and its garble by scanning electroscopic according to our research. This method can be used for identification...
The Purpose of this research is to discuss the function mechanism of raspberry ketone on simple obesity and the influence of resistance on obesity relevant insulin. There were 40 SD rats of SPF grade, and the male to female ratio was 1:1. They were randomly divided into 5 groups: NC fed with normal diet. MC fed with high-fat diet. RKL, RKM, and RKH groups fed with high-fat diet and were administered...
A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for the raspberry ketone in raspberry. Raspberry ketone was extracted by hot refluxing method with methanol as extraction solvent. The purities in crude extract was removed by chemical method, and the aldehyde, ketone and other carbonyl compounds were isolated from crude extract. Qualitative and quantitative...
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