The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that...
Reagents binding specifically to target molecules are essential tools for clinical diagnosis and targeted therapy. Screening of target cell-surface specific affinity reagents with bench-top methods has some drawbacks, including time-consuming, labor-intensive and requirement of large-scale instrument. Microfluidic platforms may overcome these drawbacks because they could automate and complete the...
In this work, we report a portable microfluidic chip based on gold nanoparticle-enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactive molecules modified on gold nanoparticles (NPs) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactive...
White blood cells or rare cells such as cancer cells with red blood cells in the whole blood, the concentration difference is approximately 1000 times in whole blood. To handle this extremely large cells population in microfluidic chip, we created a microdevice that takes advantage of the intrinsic features of blood flow in the microcirculation to separate leukocytes from whole blood, and solve clogging...
In this work, we report a portable microfluidic chip based on gold nanoparticle-enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactive molecules modified on gold nanoparticles (NPs) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactive...
The Surface Enhanced Raman Scattering (SERS) bacteria sensing chip was usually developed by wet-etching [1] or template-molding [2] approach to obtain an enhancement factor of 101−3. However, these processes may not be available for the template and difficult to integrate with other complicated fabrication designs. Therefore, in this project, we propose an easy integrated approach to develop high...
This study based on the technique, “Electrowetting on Dielectric (EWOD),” with the micro-heaters designed in EWOD microfluidics system for temperature controlling in biomedical reaction. On this platform, fluorescence magnetic beads (MBs) were used as carriers for single-nucleotide polymorphism (SNP) detection. In human genome, SNPs are responsible for the variations between individuals. The genome...
To solve clogging in microchannel and low separation rate in microfluidic devices that containing high concentration whole blood cells (4.3×106 cells/μl) with the remaining <1% consisting peripheral blood leukocytes and platelets [1–2]. In this work we introduce a like-biomimetic structure to separate red blood cells from whole blood and to enrich leukocytes (white blood cells) through the elastic...
In the proposed paper, we report dielectrophoresis (DEP) liver lab chip integrated with Poly ethylene glycol diacrylate (PEGDA) based blood vessel within the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement. The titanium (Ti) electrodes are well designed to provide positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP)...
In this work, we report a portable microfluidic chip based on gold nanoparticle-enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactive molecules modified on gold nanoparticles (NPs) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactive...
In vivo biomedical studies using animals can provide high similarity to the physiological conditions in humans. However, it is difficult to observe the molecular processes in the whole animals. On the contrary, in vitro studies could control the environmental factors in experiments but usually are difficult to reflect the complex physiological responses existing in vivo. In this paper, we report our...
A lobule-mimetic cell-patterning technique for on-chip reconstructing centimeter-scale liver tissue of heterogeneous hepatic and endothelial cells via the enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported. By mimicking the basic morphology of the liver tissue, the classic hepatic lobule, the lobule-mimetic-stellate-electrodes array is designed for cell patterning via...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.