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Insulin receptor (IR) phosphorylation is critical for the assessment of the extent of IR agonism and nuances in the downstream signaling cascade. A thorough identification and monitoring of the phosphorylation events is important for understanding the process of insulin signaling transduction and regulation. Although IR phosphorylation has been studied extensively in the past decades, only a handful...
In sporadic Alzheimer’s disease (AD), neurofibrillary lesion formation is preceded by extensive post-translational modification of the microtubule associated protein tau. To identify the modification signature associated with tau lesion formation at single amino acid resolution, immunopurified paired helical filaments were isolated from AD brain and subjected to nanoflow liquid chromatography–tandem...
In order to ensure the safety of the tunnel construction, this paper proposes the concept of tunnel deformation monitoring based on GIS, clarifies a new sets of digitalized and informatization technology to monitor tunnel deformation, discusses the principles and methods of how to develop the GIS system, gives the system structure design and explains the systempsilas function. Practice shows that...
The heat shock protein 65kDa (Hsp65) of Mycobacterium tuberculosis var. bovis was fused with the linear polypeptide epitope of cholesteryl ester transfer protein C-terminal fragment (CETPC) and expressed as soluble protein in Escherichia coli.The fusion protein Hsp65-CETPC was purified by anion exchange column and eluted at 100-130mM NaCl in 10mM phosphate buffer (pH 8.0), and then used to immunize...
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