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The heat shock protein 65kDa (Hsp65) of Mycobacterium tuberculosis var. bovis was fused with the linear polypeptide epitope of cholesteryl ester transfer protein C-terminal fragment (CETPC) and expressed as soluble protein in Escherichia coli.The fusion protein Hsp65-CETPC was purified by anion exchange column and eluted at 100-130mM NaCl in 10mM phosphate buffer (pH 8.0), and then used to immunize...
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