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This paper describes a fabrication method of muscle tissue constructs driven by neurotransmitters released from activated motor neurons. The constructs consist of three-dimensional (3D) free-standing skeletal muscle fibers co-cultured with motor neurons. We differentiated mouse neural stem cells (mNSCs) cultured on the skeletal muscle fibers into neurons that extend their processes into the muscle...
We propose a method for constructing “neuron-muscle fibers” driven by signals from activated neurons. The fibers consist of stretched muscle fibers covered with highly aligned neurons, and the axons of neurons extend into muscle fibers and form neuromuscular junctions (NMJs). We successfully constructed the neuron-muscle fibers by culturing neural stem cells on stretched muscle fibers; the neural...
This paper describes a centimeter-scale living cell fabric made of cell-containing core-shell hydrogel fibers, “cell fiber.” We improved core-shell fiber applicable to various types of cells, and precisely characterized their biofunctions and mechanical properties. Using these cell fibers, we demonstrate a centimeter-scale living cell fabric woven by our micro weaving machine. We believe that our...
We developed a method to prepare size-and shape-controlled islet-cell spheroids. We cultured beta-cell line (MIN6-m9) in PDMS-microwells and enabled to construct their spheroids with the various sizes corresponding to those of the microwells. We demonstrated the geometric analysis of insulin secretion of the cells in the spheroids. We found that these spheroids respond to glucose and subsequently...
Previously, we established a culture system of the accessory olfactory bulb in order to investigate the functional role of each accessory olfactory bulb neurons in pheromonal signal processing. In the present study, we developed a co-culture system of cultured accessory olfactory bulb neurons with partially dissociated cells of the vomeronasal organ. The dissociated cells of the vomeronasal organ...
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