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This paper reports a novel approach to scan with a high resolution the relative humidity around water in microsized environment. The humidity sensor is composed of a MEMS device, the Silicon Nano Tweezers (SNT) and a DNA bundle. The principle of the measurement is based on the relation between the electro-mechanical properties (conductance and stiffness) of a DNA bundle and the relative humidity....
Micro and nano systems (MNS) and Nano scaled devices, that are capable of handling fluids and to interact with DNA and proteins enable bio analysis at the “ultimate” molecular level and are prone to be coupled to IC Technology. This paper includes recent developments in this area, aimed at illustrating the diversity and potential of the MNS approach: (1) micromachined tweezers with sharp tips successfully...
A molecular detection system was realized using the change in the characteristics of the kinesin-coated bead motion along protein-attached microtubules. A hybrid bio/microfluidics device with a microtubule immobilized main channel and protein-injected sub-channels allowed us to compare different protein attachments and several control experiments with the same experimental conditions. As kinesin/microtubule...
In a motor protein system (kinesin/microtubule), individual microtubules were captured and relocated to build a complex nano-scale transport system. Micro tweezers, attached to a micromanipulator arm, were used to capture polarity marked microtubules. Precise relocation of those microtubules provided an oriented track along which kinesin-coated beads move according to the microtubule polarities.
We have utilized a cytoskeleton, microtubule (MT), as a template for conductive nanowires. Biotin-labeled MTs were coated with streptavidin-labeled nanogold (NG) particles. Labeling of two modified MTs, biotin-labeled and NG-coated MTs, were evaluated by fluorescent and scanning electron microscopy, which verified the specific binding of NG particles on biotin-labeled MTs. Then, NG-coated MTs were...
A lambdaDNA manipulation by motor proteins has been demonstrated by designing a fully self-assembled molecular system. Each end of lambdaDNA was modified by biotin and digoxin, respectively, based on fragment labeling and ligation methods. Digoxin-functionalized end was immobilized on a glass surface coated with anti-digoxigenin antibody. Biotinylated end was freely suspended and experienced Brownian...
Artificial nanotransport inspired by intracellular transport processes has been investigated for over a decade using the motor protein kinesin and microtubules to achieve unidirectional cargo transport. Here, we demonstrate simultaneous bidirectional nanotransport of microspheres by integrating the oppositely directed motor proteins, kinesin and dynein, with an oriented microtubule track. Our statistical...
A hybrid bio-electrical system for direct transportation and electrofusion of oil droplets is proposed. A motor protein system, kinesin/microtubule, was used to transport kinesin-coated oil droplets along microtubules oriented and immobilized on a glass surface. The attachment between the biotinylated-kinesin and the functionalized oil droplet was achieved using the biotin-streptavidin binding. Fusion...
A bio-hybrid microsystem for sorting and carrying target molecules by bio-functional molecules is presented. Different target molecules, biotin-4-fluorescein and Rabbit anti-mouse IgG labeled with Alexa Fluor 568, were selectively attached on different beads (Streptavidin-coated and protein A-coated beads). Target molecules were separately transported by a motor protein system (kinesin on beads /...
A motor protein system, kinesin/microtubule, transported target molecules specifically attached on bead surfaces. The target molecule, biotin-4-fluorescein, was specifically immobilized on streptavidin-coated beads, on which kinesin molecules were also attached. The target molecules were successfully transported along microtubules immobilized on a glass surface. The activity of kinesin was not disturbed...
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