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Surface plasmon resonance (SPR) biosensors offer the ability to monitor binding rapidly, in real time, and without any labels, while the commercial instruments available have been large in size, high-cost, and not amenable to field applications. An SPR sensor system based on Spreeta devices was developed to detect the ampicillin residues in milk for field use. Analysis conditions, including ligand...
The development of an assay for trace detection of ampicillin using an optical biosensor based on surface plasmon resonance (SPR) is reported. The biospecific interactions between ampicillin and ampicillin monoclonal antibody were monitored in real time. Water solution with a series ampicillin concentration is detected. The analysis time per sample was less than 10 minutes. Furthermore, the influencing...
The use of a surface Plasmon resonance immunosensor for the analysis of ampicillin is described. An immunoassay was developed with a miniature surface plasmon resonance (SPR) biosensor to quantify the ampicillin residues in milk samples. It was a competitive inhibition assay with ampicillin covalently immobilized on the sensor surface. A fixed amount of monoclonal anti-ampicillin antibody was mixed...
A nanoscale semiconducting ZnO based biosensor with integrated microfluidics is designed, fabricated and tested to demonstrate the detection of streptavidin, a commonly used protein. Amperometric (I-t) measurement is utilized to detect the change of conductivity over time. By comparing with the control experiment, the specific binding event between biotin and streptavidin is detected. The data indicates...
There is an increasing demand for portable, reliable, and cost effective bioelectronic systems for applications ranging from clinical diagnosis to homeland security. Conventional detection systems involve labeling the probe molecules, large amount of target molecules to enable detection, and elaborate signal transduction methods. Most of them also have to couple with optical detection equipments that...
A nanobioelectronic system-on-package (SOP) with integrated electrochemical sensors, microfluidic channels and microneedles was demonstrated with organic compatible processes. A novel amperometric glucose sensor based on carbon nanotubes/glassy carbon working electrodes and glucose oxidase enzyme encapsulated in a sol-gel derived zirconia/nafion matrix was developed to demonstrate the biosensing....
The hydrolysis of starch converting into glucose, cyclodextrins and other monomer products is very critical in food and pharmaceutical industries. The conventional methods of testing the hydrolysis products are cumbersome and time consuming. The single-crystal zinc oxide (ZnO) structures are quasi-one dimensional semiconducting nanostrucrures, and open a new field of biosensor technology for fabricating...
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