Search results for: Takeshi Kawano

Items from 1 to 20 out of 56 results

article

Long nanoneedle-electrode devices for extracellular and intracellular recording in vivo

Yoshihiro Kubota, Shota Yamagiwa, Hirohito Sawahata, Shinnosuke Idogawa, more

Sensors and Actuators B: Chemical > 2018 > 258 > C > 1287-1294

Nanowire and nanotube-based nanoscale needle devices are powerful tools for intracellular applications, including electrical intracellular recording. However, the applications of these devices to thick biological tissues, such as brain slices or brain tissue in vivo, are limited due to the short length of conventional needles (<10 μm). In this study, we fabricated 200 μm- and 400 μm-long nanoscale-tipped...

article

Visible light-responsive cell scaffolds with bilayer structures for single cell separation

Yusuke Nakajima, Takeshi Kawano, Yusuke Taki, Chie Kojima

Research on Chemical Intermediates > 2018 > 44 > 8 > 4745-4754

Collagen is a major component of the extracellular matrix, and collagen gels have been used as cell scaffolds. We previously prepared gold nanoparticle (AuNP)-embedded collagen gels (AuCol) to serve as cell scaffolds that were sensitive to visible light. We performed single cell detachment from this cell scaffold using a microscope equipped with a laser irradiation system. In the present study, we...

chapter

In vivo neuronal recordings using three-dimensional microneedle-electrode assembled on flexible substrate

Shota Yamagiwa, Hirohito Sawahata, Rika Numano, Makoto Ishida, more

2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) > 1722 - 1725

2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

Further requirements for microelectrode devices used in in vivo neuronal recordings include i) the fine needle geometry, ii) miniaturization of the device substrate, and iii) the device flexibility. This paper reports a < 10-μm-diameter three-dimensional (3D) needle-electrode assembled on a 10-μm-thick 1×2 mm² flexible film. Herein, we developed a fabrication process, in which a vapor-liquid-solid...

article

Visible Laser‐Induced In Situ Cell Detachment from Gold Nanoparticle‐Embedded Collagen Gel

Chie Kojima, Yusuke Nakajima, Naoya Oeda, Takeshi Kawano, more

Macromolecular Bioscience > 17 > 5 > n/a - n/a

Cell sorting is important for cell biology and regenerative medicine. A visible light‐responsive cell scaffold is produced using gold nanoparticles and collagen gel. Various kinds of cells are cultured on the visible light‐responsive cell scaffold, and the target cells are selectively detached by photoirradiation without any cytotoxicity. This is a new image‐guided cell sorting system.

chapter

Hook and loop microfastener: Flexible microelectrodes tied to a nerve

Yusuke Seki, Shota Yamagiwa, Yusuke Morikawa, Hirohito Sawahata, more

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS) > 117 - 120

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)

Neural recording from a nerve is a way to offer safer brain-machine interface (BMI) technology, compare to that from a brain tissue. However, because a nerve is soft and small diameter geometry, attachment of an electrode device to the nerve is still problematic, resulting in unstable recording and damage to the nerve. Here we propose a highly-flexible and -biocompatible parylene-based microelectrode...

chapter

Ultra high-aspect-ratio neuroprobe: 5-μm-diameter and 400-μm-length needle detects action potentials in VIVO

Shota Yamagiwa, Hirohito Sawahata, Hideo Oi, Rika Numano, more

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS) > 553 - 556

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)

This paper reports 5-μm-diameter and 400-μm-length ultra high-aspect-ratio needle electrodes for neuronal action potential recordings in vivo. The needle-electrode was vertically assembled on a 1 × 1 mm² block module of silicon by vapor-liquid-solid (VLS) silicon growth technology for ∼ 7 hours. After metallization and encapsulation of the needle, the module device was packaged for in vivo animal...

article

Corrigendum to <Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells> <Cancer Lett. 340 (1) (2013) 82–87>

Masaharu Akiyama, Kohji Ozaki, Takeshi Kawano, Osamu Yamada, more

Cancer Letters > 2016 > 380 > 2 > 608

chapter

Micro/nanoscale needle technology for brain

Takeshi Kawano

2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO) > 933 - 936

2016 IEEE 16th International Conference on Nanotechnology (IEEE-NANO)

This paper reports our recent progresses on silicon growth technology-based micro/nanoscale three-dimensional (3D) needle-electrode array devices, for use in electrophysiological recordings of neurons/cells within the brain.

chapter

3D microtube and 2D flexible film waveguide devices

Takeshi Kawano

2016 International Conference on Optical MEMS and Nanophotonics (OMN) > 1 - 2

2016 International Conference on Optical MEMS and Nanophotonics (OMN)

This paper reports microscale three-dimensional (3D) tube-like waveguide array and two-dimensional (2D) flexible waveguide array devices. Such microdevices will offer a new class of optogenetic applications, including light stimuli for specific regions of a neuron (e.g., axons or dendrites) and numerous spherical bio-tissues (e.g., brain and spinal cord).

article

Nanoscale‐Tipped High‐Aspect‐Ratio Vertical Microneedle Electrodes for Intracellular Recordings

Yoshihiro Kubota, Hideo Oi, Hirohito Sawahata, Akihiro Goryu, more

Small > 12 > 21 > 2846 - 2853

Intracellular recording nanoscale electrode devices provide the advantages of a high spatial resolution and high sensitivity. However, the length of nanowire/nanotube‐based nanoelectrodes is currently limited to <10 μm long due to fabrication issues for high‐aspect‐ratio nanoelectrodes. The concept reported here can address the technological limitations by fabricating >100 μm long nanoscale‐tipped...

article

Intracellular Electrodes: Nanoscale‐Tipped High‐Aspect‐Ratio Vertical Microneedle Electrodes for Intracellular Recordings (Small 21/2016)

Yoshihiro Kubota, Hideo Oi, Hirohito Sawahata, Akihiro Goryu, more

Small > 12 > 21 > 2803 - 2803

The length of nanowire/nanotube‐based nanoelectrodes is currently limited to <10 μm due to fabrication issues for high‐aspect‐ratio nanoelectrodes. On page 2846, T. Kawano and co‐workers demonstrate how a fabricated microneedle‐based nanoscale‐tipped electrode, over 100‐μm long, can penetrate into muscle cells and observe the resting potentials from cells.

chapter

An origami-inspired ultrastretchable bioprobe film device

Yusuke Morikawa, Shota Yamagiwa, Hirohito Sawahata, Makoto Ishida, more

2016 IEEE 29th International Conference on Micro Electro Mechanical Systems (MEMS) > 149 - 152

2016 IEEE 29th International Conference on Micro Electro Mechanical Systems (MEMS)

We have developed an ∼1,000% stretchable and flexible bioprobe film device, which enables to follow the shape of a spherical and deformable biological tissue while the electrical signals are recorded (Fig. 1). As the film material, we use a highly-biocompatible parylene film, which shows the high stretchability by embedding slit patterns in the film, so call ‘Amanogawa’ design in traditional ‘Origami...

chapter

High-performance microelectrode of PEDOT/Pt-black for low voltage neurostimulaiton

Kentaro Yamaguchi, Masanori Tanaka, Shota Yamagiwa, Hiroto Sawahata, more

2016 IEEE 29th International Conference on Micro Electro Mechanical Systems (MEMS) > 710 - 713

2016 IEEE 29th International Conference on Micro Electro Mechanical Systems (MEMS)

Here we report a material for low-voltage and micro-scale electrode stimulation, that combines high charge injecting and low impedance characteristics of poly (3, 4-ethylenedioxythiophene) (PEDOT) with a large effective surface area by using a platform of platinum black (Pt-black). The layer-by-layer assembled PEDOT/Pt-black electrode with the diameter of 30 μm exhibits the charge-injection delivery...

article

Impact of Fukushima-derived radiocesium in the western North Pacific Ocean about ten months after the Fukushima Dai-ichi nuclear power plant accident

Yuichiro Kumamoto, Michio Aoyama, Yasunori Hamajima, Akihiko Murata, more

Journal of Environmental Radioactivity > 2015 > 140 > Complete > 114-122

We measured vertical distributions of radiocesium (¹³⁴Cs and ¹³⁷Cs) at stations along the 149°E meridian in the western North Pacific during winter 2012, about ten months after the Fukushima Dai-ichi Nuclear Power Plant (FNPP1) accident. The Fukushima-derived ¹³⁴Cs activity concentration and water-column inventory were largest in the transition region between 35 and 40°N approximately due to the directed...

chapter

Vertically aligned extracellular microprobe arrays/(111) integrated with (100)-silicon mosfet amplifiers

Hiroki Makino, Kohei Asai, Masahiro Tanaka, Shota Yamagiwa, more

2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS) > 686 - 689

2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

We report a heterogeneous integration of vertically aligned extracellular micro-scale silicon (Si)-probe arrays/(111) with MOSFET amplifiers/(100), by IC processes and subsequent vapor-liquid-solid (VLS) growth of Si-probes. To improve the extracellular recording capability of the microprobe with a high impedance of > 1 MΩ at 1 kHz, here we integrated (100)-Si source follower buffer amplifiers...

article

Layer-by-layer assembled nanorough iridium-oxide/platinum-black for low-voltage microscale electrode neurostimulation

Shota Yamagiwa, Akifumi Fujishiro, Hirohito Sawahata, Rika Numano, more

Sensors & Actuators: B. Chemical > 2015 > 206 > Complete > 205-211

Electrical neural stimulating electrodes play an important role in medical applications and improving health/medical conditions. However, size reduction for low-invasive electrodes creates issues with high electrolyte/electrode interfacial impedance and low charge-injection characteristics, which makes it impossible to stimulate neurons/cells. To overcome these limitations, we propose an electrode...

chapter

Micro-electrode arrays for multi-channel motor unit EMG recording

Shota Yamagiwa, Hirohito Sawahata, Makoto Ishida, Takeshi Kawano

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) > 857 - 860

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)

We report an array of micro-electrodes, which can record multi-channel motor unit (MU) electromyogram (EMG) signals with a high spatial resolution. As a basic structure of the electrode, we fabricated an array of 200-μm-square Si-pyramids with the height of 200 μm by Tetramethylammonium hydroxide (TMAH), in order for robust MU-EMG recordings without conductive gel. Platinum (Pt) was used as the electrode...

chapter

A vertically integrated nanoscale tipped microprobe intracellular electrode array

Yoshihiro Kubota, Hideo Oi, Hirohito Sawahata, Akihiro. Goryu, more

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) > 155 - 158

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)

Here we report integration of nanoscale tipped 120-μm-long vertical microprobe electrode (NTE) array and intracellular recordings using a gastrocnemius muscle of a mouse. The tip diameter and curvature radius of the NTE was < 200 nm, respectively, and the controlled height of the exposed tip section was 4 μm. The impedance of the fabricated NTE exhibited 3.1 MΩ at 1 kHz in saline, with the output/input...

article

Flexible Neural Electrode Arrays With Switch-Matrix Based on a Planar Silicon Process

Akifumi Fujishiro, Sou Takahashi, Kazuaki Sawada, Makoto Ishida, more

IEEE Electron Device Letters > 2014 > 35 > 2 > 253 - 255

Herein, we fabricate a flexible microelectronic system using a conventional silicon (Si) integrated circuit process. The fabricated device is a ${<}{\rm 12}\hbox{-}\mu{\rm m}$ -thick film flexible 7 $\,\times\,$ 8 (56 ch) switch-matrix microelectrode array, which can be used to record the electrical activity from numerous three-dimensional biological tissues. The embedded Si-nMOSFETs/(111) in a polyimide...

article

Phosphoglycerate kinase deficiency due to a novel mutation (c. 1180A>G) manifesting as chronic hemolytic anemia in a Japanese boy

Masato Tamai, Takeshi Kawano, Ryota Saito, Ken Sakurai, more

International Journal of Hematology > 2014 > 100 > 4 > 393-397

Phosphoglycerate kinase (PGK) deficiency, a rare X-linked inherited disorder, manifests as various combinations of hemolytic anemia, neurological dysfunction, and myopathy. We report a Japanese boy with PGK deficiency presenting as chronic hemolytic anemia. The diagnosis of PGK1 deficiency was made at 11 months of age on the basis of low PGK enzyme activity (36.7 IU/g Hb; normal, 264–326 IU/g Hb)...

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HUMAN TELOMERASE REVERSE TRANSCRIPTASE (3)
IMPEDANCE (3)
IN VIVO (3)
INTRACELLULAR RECORDING (3)
NEURON (3)
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K562 (2)
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NEURAL INTERFACE (2)
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RETINOBLASTOMA (2)
STI571 (2)
VLS GROWTH (2)
PARTIAL PRESSURE OF CO 2 (1)
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134CS (1)
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INFONA - science communication portal

Search results for: Takeshi Kawano

Long nanoneedle-electrode devices for extracellular and intracellular recording in vivo

Visible light-responsive cell scaffolds with bilayer structures for single cell separation

In vivo neuronal recordings using three-dimensional microneedle-electrode assembled on flexible substrate

Visible Laser‐Induced In Situ Cell Detachment from Gold Nanoparticle‐Embedded Collagen Gel

Hook and loop microfastener: Flexible microelectrodes tied to a nerve

Ultra high-aspect-ratio neuroprobe: 5-μm-diameter and 400-μm-length needle detects action potentials in VIVO

Corrigendum to <Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells> <Cancer Lett. 340 (1) (2013) 82–87>

Micro/nanoscale needle technology for brain

3D microtube and 2D flexible film waveguide devices

Nanoscale‐Tipped High‐Aspect‐Ratio Vertical Microneedle Electrodes for Intracellular Recordings

Intracellular Electrodes: Nanoscale‐Tipped High‐Aspect‐Ratio Vertical Microneedle Electrodes for Intracellular Recordings (Small 21/2016)

An origami-inspired ultrastretchable bioprobe film device

High-performance microelectrode of PEDOT/Pt-black for low voltage neurostimulaiton

Impact of Fukushima-derived radiocesium in the western North Pacific Ocean about ten months after the Fukushima Dai-ichi nuclear power plant accident

Vertically aligned extracellular microprobe arrays/(111) integrated with (100)-silicon mosfet amplifiers

Layer-by-layer assembled nanorough iridium-oxide/platinum-black for low-voltage microscale electrode neurostimulation

Micro-electrode arrays for multi-channel motor unit EMG recording

A vertically integrated nanoscale tipped microprobe intracellular electrode array

Flexible Neural Electrode Arrays With Switch-Matrix Based on a Planar Silicon Process

Phosphoglycerate kinase deficiency due to a novel mutation (c. 1180A>G) manifesting as chronic hemolytic anemia in a Japanese boy

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