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In this study, we examined the expression of hWAPL in cervical intraepithelial neoplasia (CIN) I-III and cervical squamous cell carcinoma, and investigated clinical application value of hWAPL in diagnosis of cervical disease. 90 samples were collected, and two slides from each sample were immunostained using hWAPL and p16INK4a antibody. HPV-DNA testing was performed using the HC2 DNA test. The expression...
To investigate the effects of different doses of X-ray on the telomerase in human osteosarcoma (HOS) cells and to provide theoretical evidence for the clinical application of radiotherapy in osteosarcoma. Log phase HOS cells were radiated using 1, 2, 4 and 8 Gy doses of X-ray separately and cell survival was measured using MTT method, telomerase activity by ELISA, and telomerase reverse transcriptase...
The expression of TNF super family member often undergoes post-translation modifying, including internalization and shedding by specific enzymes. Matrix metalloproteases (MMPs) and proteases containing a disintegrin and a metalloprotease domain (ADAMs) are often responsible for the shedding of these members. But the proteases shedding TNF-related apoptosis inducing ligand (TRAIL, a newly described...
To help the biomedical scientist pre-confirm the disease-related genes, we considered these gene as a whole research set and analyzed the topological features of their interaction network. Two strategies had been proposed to construct the disease-related gene network from the OMIM database. Using these two constructed sets, we trained two support vector machine prediction models, the accuracy of which...
Okadaic acid (OA), a key diarrheic shellfish poisoning (DSP) toxin will possibly arouses DSP symptoms by consuming the contaminated shellfish. The DSP toxins are stable at high temperatures, and long-term DSP toxicity is carcinogenic. Therefore a fast and reliable analytical method for the detection of OA and analogues in shellfish is worth developing. In this paper, a direct competitive ELISA (dcELISA)...
We aimed to investigate the expression and location change of ER-a36 in BGC823 when dealt with TAM. Cell growth was examined by WST-1 and assayed by microplate reader. ER-a36 expressions in mRNA and protein were assayed by RT-PCR and Western-blot method and were quantitated. The location of ER-α36 in cell was examined by immunofluorenscence methods. The cell numbers significant decreased at all TAM...
To investigate the role of the angiopoietin-2 (Ang-2) signal transduction pathway in colon cancer, colon cancer cells (SW1116) were divided into four groups, control group, serum-free group, Ang-2 group (treated with 1.2 mg/L Ang-2) and PI3K/Akt inhibitor group (treated with LY294002 and Ang-2). Then Tie-2, PI3K and Akt were detected via Western blotting after 24 h. Compared to the serum-free group,...
Objective: To study the expression of angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) and the angiopoietin receptor (Tie-2) in colorectal cancer tissue, and analyze the correlation between their expression levels in colorectal cancer tissue and the histological differentiation of colorectal cancer; and to discuss their roles in the progression of colorectal cancer to provide new targets for the clinical...
GHGKHKNK octapeptide has tumoricidal potential mainly due to the amino acid residues of His-Gly-Lys motif inhibits the clone formation, adhesion and invasion of tumor cells. However, its clinical application is limited by its short half-life time in vivo. We used human serum albumin (HSA) fusion technology to fuse GHGKHKNK octapeptide and HSA to prolong half-life time of GHGKHKNK octapeptide and increase...
Lymphatic metastasis is one of the critical metastatic routes of malignant tumors, and the involvement of lymph nodes is usually one of the key predictors of prognosis. In recent years, studies on animal models and clinical studies have suggested that vascular endothelial growth factor (VEGF)-C/VEGF-D/VEGF receptor-3 (VEGFR-3) signaling system plays important roles in the process of lymphatic metastasis...
In order to explore the effect of paclitaxel (PTX) combined with Survivin siRNA on proliferation and apoptosis in U-87MG cells in vitro, the U-87MG cells were transfected with Survivin siRNA and treated with PTX at the same time. The cell morphological changes were determined by methylene blue solution. Survivin, cyclin D1, c-Myc and CDK4 expression in U-87MG cells was analyzed by RT-PCR and western...
In the present study, we investigated the effect of ERK1/2 and JNK signaling pathway in the inhibition of AcSDKP on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts induced by PDGF. By using MTT assay and Western Blot analysis, we found that treating the fibroblasts with PDGF, phospho-ERK1/2 and phospho-JNK expression was significantly increased compared with the control...
The current study showed that safflower yellow pigment can protect the endothelial cells, reduce the incidence of coronary restenosis, but the specific mechanisms have not been reported. Objective: To establish in vitro hemolytic blood lecithin-induced endothelial cell models of restenosis and of safflower yellow pigment on the vascular endothelial cell protection. Methods Cultured human umbilical...
To study the effect of β-ray irradiation on apoptosis in human benign hyperplastic prostate tissue. 20 BPH patients were randomly divided into 4 groups, no irradiation was applied in the control group, 90Sr/90Y β-ray individually irradiated the other 3 groups for 1 day, 4 days and 7 days. The apoptosis of human hyperplastic prostate tissues were studied by TUNEL detection, flow cytometry and agarose...
This paper shows a graph based method to analyze proteomic expression data. The method allows the prediction of the expression of genes not measured by the gene expression technology based on the local connectivity properties of the measured differentially expressed gene set. The prediction of the expression jointly with the stability of this prediction as a function of the variation of the initial...
We report on (a) a novel procedure to dope ferric oxide primary particles with GRAS ligands (b) their characterization and stability in cell culture solution (c) their uptake and utilization by gut cells in culture and (d) their delivery to human volunteers. Overall, these materials are cheap to manufacture and show promise for safe and efficient oral iron delivery to help ease the global burden of...
The nanomonitors are multi scale “point-of care” devices comprised of high density nanopores formed from the integration of nanoporous alumina membranes onto metallic microscale platforms. The nanomonitors have approximately 250,000 nanowells per sensing site and use antibody binding reactions for detection of proteins. The antigen explored was C-reactive protein (CRP), an inflammatory biomarker associated...
The majority of cellular functions can rarely be attributed to a single protein, gene or enzyme. Protein-protein interactions are fundamental to most of cellular processes or functions. Hubs, proteins with high connectivity, mediate the organization and function of cellular protein interaction networks (PINs). As a first step, research on hub proteins starts with classification. Although there are...
Comparative analysis of protein interaction networks plays a key role in the study of evolution and molecular etiology of diseases. A topology-based method is proposed in this paper to search biologically significant sub networks in protein interaction networks with relatively high accuracy. Different with other methods, the present method takes into account incorrectness and incompleteness of biological...
A portable leukocyte differential count is especially desirable for the emerging needs from bedside and NASA spaceflight healthcare. However, previous efforts to miniaturize the test using microfluidics have shown only a 3-part differential of leukocyte including lymphocyte, monocyte, and neutrophil, whereas the 5-part differential is still lacking. Here we report a complete 5-part differential leukocyte...
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