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For medical applications of cell sorting, counting or flow cytometry, focusing of biological cells in microdevices is a prerequisite. Hydrodynamic focusing by sheath flow has been a widely used method for particle focusing, however, additional buffer inlets and precise flow control were needed. An insulator-based dielectrophoretic microdevice of four insulating structures forming X-pattern with effective...
In this paper, we report on an on-chip separation device that is based on guided dielectrophoresis (DEP). The simple design comprises a pair of electrodes and a microfluidic channel for sample delivery. Continuous separation into two distinct populations (corresponding to positive and negative DEP, respectively) in the microchannel was achieved without the need of accurate hydrodynamic focusing or...
MEMS, electromagnetic field is a way to manipulate droplets, beads or cells without direct contact. In particular, dielectrophoresis is often used in bioMEMS applications, for cell handling operations such as moving, sorting, trapping or deforming cells. In this paper, a 3D focusing geometry for the electrical field is proposed, that permit the trapping of single cell, or cells pair, thanks to positive...
This paper presents a microfluidic chip with arc-shape electrodes array (ASEA) for separation of different-size particles/Cells based on negative dielectrophoresis (nDEP). The purpose of ASEA is to generate the dielectrophoretic force perpendicular to the microchannel direction within the gap between each pairs of arc-shape electrodes. The DEP chip consisted of two regions of ASEA; first ASEA was...
Advances in microfabrication have introduced new possibilities for automated, high-throughput biomedical investigations and analyses. Physical effects such as dielectrophoresis and electroporation can be used to manipulate particles in solution to coordinate a sequence of bioanalytical processing steps. Dielectrophoresis is accomplished with non-uniform electric fields that can polarize particles...
In this paper we describe our work that uses acoustics and dielectrophoresis to move particles/cells in microsystems. These particle manipulation technologies are used to create a high throughput system for spatiotemporal analyses of signaling within biological cells. The fabrication scheme and the focusing characteristics of the system are presented. The system allows the focusing characteristics...
This study presents the design, simulation, fabrication and measurement of a microfluidic chip with micro cylindrical post array for separating particles. The structure of microfluidic chip is consisted of top glass, a micro channel with cylindrical post array, two Au end electrodes and bottom glass. Both in-line and staggered arrays of micro cylindrical posts were designed and made of SU-8 inside...
For most orbital maneuvers, small satellites in the sub-10 kg range require thrusters capable of spanning the micro-Newton to milli-Newton force range. At this scale, electrokinetic (EK) pumping offers precise metering of monergolic or hypergolic liquid propellants under purely electrical control at pressures and flow rates well-suited to microthruster applications. We have demonstrated direct and...
Commonly, microelectrodes used in microfluidics are made from copper, Au or Pt. They are difficult to fabricate and the cost is relatively high. Thus, we designed and fabricated a novel microelectrode made from silicon for it is cheap and easy to fabricate. It has been proven that cells can be manipulated by dielectrophoresis force producing on interdigitated electrodes. In addition, the direction...
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