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Imaging bio-molecules especially in tissues, cells and even in in-vitro live conditions require expertise not only in staining and tagging of the particular molecules of interest but also their imaging so as to make any meaningful analysis and quantification of the obtained images. This necessitates a microscopic facility which can grab fluorescent images with minimum bleaching of the dyes and gives...
The functions of cell plasma membranes strongly rely on their physicochemical properties, which determine the membrane structure, control the transport of molecular species, and determine the folding and function of membrane proteins. Among these properties, the membrane electrostatics, phase state, hydration, and dynamics are of particular importance and their monitoring appears critical to further...
Chloroethylnitrosoureas (CENUs) were an important family of anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicated that their cytotoxicity was related to the interstrand crosslink of DNA. In this study, a method for the determination of DNA crosslink was established using real-time fluorescence quantitative PCR. The DNA crosslink ratio induced by two...
Many studies have showed that peroxynitrite-mediated injury on fibrinogen has some relations with Thrombotic diseases. However there are few reports about structural changes of nitrified fibrinogen when metallic ions exist in this reaction. In this study, we use methods of FTIR-ATR spectra and 3D Fluorescence spectra to study the structural changes on fibrinogen after nitration and oxidation damages...
A new concept aiming to improve surgical outcome by providing real-time surgical feedback on tumor margin delineation is presented. We are using a novel multispectral imaging system for real time measurement of fluorescence probes with molecular specificity to tumor biomarkers. Mice bearing xenograft tumors were used to simulate surgical operations of tumor excision guided by real time fluorescence...
Malignant gliomas are associated with poor prognosis. Photodynamic therapy (PDT), relying on light-activated toxicity of a photosensitizer, has been investigated as a means for improving patient survival. This review presents a summary of clinical results obtained with PDT using different photosensitizers for treating brain malignancies. Particular emphasis is on the use of 5-aminolevulinic acid (ALA)...
In the past decade, rapid development happened in the field of nanotechnology. Recently, nano-material, especially inorganic hybrid molecule, has become an increasingly hot issue in medicine area for safe drug delivery system. In this paper, nano-layered double hydroxides (nano-LDHs) were chosen to be a potential drug carrier. In order to explore the biocompatibility of nano-LDHs with Hela cells,...
DNA microarrays are typically composed of micron-scale spots of specific genetic sequences attached to a glass microscope slide for the purpose of gene expression analysis. By replacing the glass microarray substrate with a photonic crystal surface, fluorescence enhancement effects can be utilized to improve the performance of the microarray. This work describes the design of photonic crystal surfaces...
The binding of efonidipine to bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and circular dichroism (CD) technique. The quenching mechanism of fluorescence BSA by efonidipine showed that efonidipine quenched the fluorescence of tryptophan residue mainly through the collision mode. The thermodynamic parameters DeltaH0 and DeltaS0 were calculated to be 75.95 kJldrmo-1 and 342...
Several artificial intelligent methods, including support vector regression (SVR), artificial neural networks (ANNs), and partial least square (PLS) are used for the multivariate calibration in the determination of the three aromatic amino acids (phenylalanine, tyrosine and tryptophan) in their mixtures by fluorescence spectroscopy. The results of the leave-one-out method show that SVR perform better...
Initial results of a comprehensive design software that optimizes parameters for fluorescence analysis of a user-defined fluorophore are presented. SLAP (spectral LED aggregation program) automatically selects configurations of LEDs that, in a fluorescence analysis system, maximize the emission signal (useful output) as a function of the excitation signal (interference), optics, photodetection modality,...
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