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4-Mercaptobenzoic acid coated gold nanoparticles were used as Raman based labels in a standard immunoassay for mouse IgG. The Au nanoparticles used had a diameter of 51 ± 2 nm and were synthesised by the citrate reduction method. Dynamic light scattering and TEM image analysis were used to characterise the nanoparticles. The SERS spectra was recorded on an EZRaman desk-top spectrometer operating with...
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement,...
In this paper, an optofluidic device was reported for the reproducibility and sensitivity improvement of surface enhanced Raman spectroscopy (SERS) for molecular detection. The optofluidic device can trap nanoparticles and molecules at a specific location to form nanoparticles/molecules clusters with reliable and hot spot site. With these advantages, this technique enables the early detection of diseases...
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