The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
Predicting of Human Leukocyte Antigen (HLA) gene can provide procedure into the human immune system. The classification of HLA genes has been developed by using various computational methods random forest based on codon usage. And ten-fold cross-validation to evaluate the models. Here, we propose methods of amino acid composition (AAC), dipeptide compositions (DPC) and p-collocated to investigate...
Background: Helicobacter pylori (H.pylori) is one of the most common chronic infections worldwide. The H. pylori Urease is a high molecular mass (530 kDa) multimeric enzyme collected of two separate subunits, UreA (26.5 kDa) and UreB (61.7 kDa). Recently, many researchers are working in the expansion of a vaccine to prevent H. pylori infection, and of the various candidate antigens, the majority promising...
Malaria is one of the most deadly infectious diseases in the world. The malaria burden is characterized by 207 million cases and over 627,000 deaths annually. The consistent morbidity and mortality underscore an urgent need for the development of next-generation antimalarials. In this paper, we propose a network mining approach to uncover the protein-protein associations that are implicated in important...
We propose a method that can be used to quantitatively measure concentrations of protein through a semiconductor sensor with a robust signal. Measurement of protein by a semiconductor may encounter a major problem. For specifically measuring protein, an antibody is immobilized on the semiconductor sensor surface. The physical length of the antibody is around 10 nm, a distance too long to be sensed...
The 21st century has embraced miniaturization and witnessed the emergence of multidisciplinary nanotechnology in the medical field, popularly known as nanomedicine. It has long been established that chronic infections such as hepatitis and malaria, as well as diseases such as cancer and arthritis, are diverse, complex, and heterogeneous, wherein patients do not respond identically to the same spectrum...
Thirty-four million people are living with HIV worldwide, a disproportionate number of whom live in resource-limited settings. Proper clinical management of AIDS, the disease caused by HIV, requires regular monitoring of both the status of the host's immune system and levels of the virus in their blood. Therefore, more accessible technologies capable of performing a CD4+ T cell count and HIV viral...
Glucocorticoid (GC) resistance is a key mechanism by which traditional asthma treatments become ineffective for patients, yet the molecular characteristics of the associated regulatory changes are largely unknown. Significant evidence suggests that crosstalk between p38 Mitogen Activated Protein Kinase (MAPK) and GC signalling pathways may contribute to this resistance. Based on a number of studies,...
Protein therapeutics is used in the treatment of diabetes and various forms of cancer. A major concern is that repeated administration to patients often leads to undesirable antidrug antibodies with a wide range of life threatening consequences that induce immunogenicity or an adverse response of the immune system. The antibodies are generally thought triggered by the tendency of monomer protein molecules...
Immunoassay for determining aflatoxin B1 involves in using the toxic mycotoxin in a conjugated form. To replacing the toxic conjugate in an immunoassay, four mimotope peptides of aflatoxin B1 were acquired by a panning-elution selection from a phage library, and one mimotope peptide was fused with the major coat protein gVIIIp by the pC89 phagemid display system. It led to a high copy number expression...
Immunisation against H. pylori is an attractive option for antibiotic resistance and reinfection situations. Strain genetic heterogeneity, and low immunogenicity of protein antigens and DNA alone are nonetheless obstacles to this approach. We developed multigenic H. pylori DNA-nanoparticle and protein-nanoparticle vaccines based on pathogenic relevance. Six antigens were chosen for the vaccine construction:...
Helicobacter pylori is responsible for several gastric diseases. The main constraints of vaccine trials against this pathogen are mainly due to the bacterium high antigenic variability and to down-regulation of the host immune responses. To counteract these factors we propose a DNA vaccine able to induce a balanced humoural and citotoxic specific immune responses, based on multi-antigens. The selection...
To construct a hp0532 gene-deleted mutant of Helicobacter pylori and study its impact on translocation of CagA protein. A recombinant fragment containing a kanamycin resistance gene that was flanked by the up- and down-stream portions of the gene hp0532 was amplified and engineered into pBluescript SK II-plasmid. To construct suicide plasmidpBlueKM40-/lhp0532 based on allelic exchange, Electrotrans...
Legionella pneumophila is an intracellular bacteria, and its successful parasitism in host cells involves two reciprocal phases: transmission and intracellular replication. In this study, we try to construct expression vectors of pcDNA3.1 (+)-lvgA/ctxB-EGFP fused gene and to investigate its transient expressions in NIH3T3 cells. Then preliminary analysis the immunogenicity of lvgA/ctxB fusion gene...
In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS-PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein...
For raising the antigenicity of Mycobacterium bovis single antigen, fusion protein of two genes was acquired. The DNA fragments of mpb83 and ag85b were fused by splicing by overlapping extension (SOE) polymerase chain reaction(PCR), and the fusion gene mpb83-ag85b were cloned into pMD18-T vector, then we got the recombinant plasmid pMD-83-85b. pMD-83-85b and pET28a(+) were digested by BamH I and EcoR...
[Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. [Method] A pair of primers was designed according to nucleotide sequences of cold resistant gene CAS19 of M. sativa, and then RT-PCR was used to amplify the protein gene of CAS19, which was then cloned into pMD18-T vector and subcloned into expression vector PBI121. The recombination...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.