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This study proposed a Polydimethylsilcoxane (PDMS) concentration gradient microfluidic chip fabricated using MEMS, casting molding, and microimprinting technology. The chip is used to research on optimizing concentration of endothelial cells on glucose concentration gradient is extracted using this chip. In the experiment a concentration gradient in solution was generated by a microfluidic network,...
It is recognized that topographical features such as ridges and grooves can dramatically influence cell phenotype, motivating the development of substrates with precisely biomimetic topography for study of the influence on cultured cells. Intestinal basement membrane topography has been precisely replicated using plasma enhanced chemical vapor deposition (CVD) of poly(2-hydroxyethyl methacrylate)...
We have utilized a plasma based patterning technique, that we have developed, to study the effects of micrometer and submicrometer scale surface functionalization on cell proliferation and morphology. We have applied this method to cellular patterning and examined the response of mammalian cells to patterns of hydrophobic and hydrophilic areas created directly on polystyrene substrates. We have observed...
This paper describes a method for handling a single adherent cell using a self-assembly technique. We produce microplates using Parylene with both hydrophilic and hydrophobic sides. We culture the cells on the hydrophilic side, and utilize the hydrophobic side to self-assemble the plates onto the hydrophobic regions. Culturing a single cell on each Parylene microplate facilitates their handling since...
Molecular motors are compact, efficient and sophisticated micro-/nanoscale actuators. However, the process of isolating molecular motors from microorganisms has a low yield; moreover, providing the isolated motors with energy as well as controlling them is very challenging. We have previously demonstrated that flagellar motors within the intact cells of Peritrichous bacteria can be used for controlled...
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