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We report the synthesis and characterization of multi-color gold nanoparticle based tags (Raman nanotags) for molecular imaging. The multi-color Raman nanotags are PEGylated gold nanoparticles (AuNPs) encapsulating a Raman reporter dyes which can be functionalized with any ligand of interest for targeted molecular imaging. The Raman nanotags synthesized with 65 nm gold nanoparticles exhibit the largest...
In this paper, we present a ultra high sensitive (Zeptomole, 10-21) technique to enable the detection of any potential low abundance biomarkers. We demonstrated for the first time the detection of sub 13 nm high-moment magnetic nanoparticle and the implementation of a novel magnetoresistive (GMR) biosensor concept with higher sensitivity and 10 times lower external field in real biomarker sensing...
The effectiveness of an imaging technique is often based on the ability to image quantitatively both morphological and physiological functions of the tissue. Here we present several ultrasound-based imaging techniques capable of visualizing both structural and functional properties of living tissue. Each imaging system utilizes custom-made, targeted nanoparticles developed to probe specific molecular...
Optoacoustic techniques allow imaging of tissue structures with optical contrast and acoustical resolution. This modality is ideal for visualization of blood vessels since haemoglobin is one of the best-absorbing tissue chromophores. It therefore can provide vasculature images with much higher contrast than pure ultrasound. If compared with standard techniques for blood imaging such as Doppler ultrasound,...
In order to achieve an efficient biolabeling, a reliable marker is needed. Organic dyes and fluorophores are widely used, but they lack of photophysical stability. Alternative systems are Quantum dots (QDs). Although very efficient for multicolour staining and no photobleaching, they suffer of photoblinking and may be cytotoxic in long term. In contrast, diamond nanoparticles (NDs) containing nitrogen-...
In order to purify blogical Chinese herbal medicine from wood etractives and hmicellulose at the molecular level, the fresh Chinese-fir cells were extracted in LiOH solution, and were analyzed by ZETA and SIZER to find out the leaching rule of nano particles in Chinese-fir cells. The results are as follows: (1) The extraction mass arrived at the maximum value when LiOH concentration is about 17.5%...
Cartilage damage is difficult to repair,as a major stimulator of matrix synthesis in cartilage, insulin-like growth factor I (IGF-I) is likely to play a important role in the process of repairing cartilage damage. To study the in vivo therapeutic effect of chitosan mediate igf-lgene transfection on repairing articular cartilage defect in rabbits.Chitosan-DNA nanoparticlesCS/igf-1 were prepared following...
Nanoparticles with various formations were produced based on ionic gelation process between chitosan and tripolyphosphate (TPP) by using bovine serum albumin (BSA) as a model drug. Their average diameter was 300-700 nm with the examination of laser granularity analyzer. The morphologies were examined with optical microscope and TEM. FTIR was used to study the structure characterization between chitosan...
In the past decade, rapid development happened in the field of nanotechnology. Recently, nano-material, especially inorganic hybrid molecule, has become an increasingly hot issue in medicine area for safe drug delivery system. In this paper, nano-layered double hydroxides (nano-LDHs) were chosen to be a potential drug carrier. In order to explore the biocompatibility of nano-LDHs with Hela cells,...
The application of cellulases in various industries demands highly stable enzymes, able to perform at extreme pH values and temperatures. In this study, the cellulase, which was immobilized on the chitosan-coated magnetic nanoparticles modified with alpha-ketoglutaric acid (alpha-KA-CCMNPs), exhibited a broader pH range of high activity and the loss of the activity of immobilized cellulase was lower...
A novel optical biosensor assembled with gold colloidal nanoparticles on gold plate surface has been constructed via 1,4-dithiothreitol and L-cysteic acid for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The prepared nano gold sol were characterized by using UV-Vis absorption spectroscopy,...
We have utilized a cytoskeleton, microtubule (MT), as a template for conductive nanowires. Biotin-labeled MTs were coated with streptavidin-labeled nanogold (NG) particles. Labeling of two modified MTs, biotin-labeled and NG-coated MTs, were evaluated by fluorescent and scanning electron microscopy, which verified the specific binding of NG particles on biotin-labeled MTs. Then, NG-coated MTs were...
In this work, we develop a platform for assaying biomolecules involving the measurement of alternating current AC magnetoreduction of magnetic reagent mixed with a detected sample. The magnetic reagent contains magnetic nanoparticles coated with a given kind of antibody, which associates with conjugated biomolecules. Then, the biomolecules can be quantitatively detected by measuring the AC magnetoreduction...
To explore the nanoparticles-dependent signaling pathways is a rapidly growing research field. Here, we globally elucidate the regulation modules of cellular molecules by using graph-based representation of complex networks. We have packed some useful bioinformatics methods (Matlab Simbiology and Pathway Studio) into an integrated system for graph-based representation and complex networks modeling...
Zirconia particles were introduced into a methyl methacrylate monomer (MMA) liquid and polymethyl methacrylate (PMMA) beads during polymerization to develop novel bone cement substrates. Osteoblasts (bone-forming cells) were seeded at a density of 3500 cells/cm2 and were cultured on unmodified bone cements comprising of only PMMA, bone cements modified with micron ZrO2 additives, bone cements modified...
The physicochemical context in which molecules are presented at the surfaces of microbes has tremendous implications for the immune response to vaccination. The spacing and mobility of molecules may control interactions of their receptors, influencing immune cell activation, pathogen uptake, and antigen processing. The chemical environment of antigens also influences the specificity of the humoral...
A hybrid, nanosized, localized and release-controlled bone morphogenetic protein delivery system consisting of a liposomal core incorporated into a shell of alternating layer-by-layer self-assembled natural polyelectrolytes has been formulated. Hydrophilic, monodisperse, spherical and stable cationic nanoparticles with an extended shelf-life allowing immediate protein loading prior to clinical administration...
We demonstrate a miniaturized AC magnetic susceptometer to detect biomolecules tagged to magnetic nanoparticles (MNPs). The proposed compact susceptometer measures the magnetic relaxation spectrum of biomolecules tagged with MNPs in an aqueous suspension. The relaxation time corresponds to the Brownian relaxation time and depends on the hydrodynamic size of the MNPs. Conventional susceptometers are...
Bacteriophage phi29 DNA packaging motor is geared by a six-pRNA ring. pRNA is able to form a multimeric complex and patterned superstructures via the interaction of two reengineered interlocking loops. This unique feature makes it an ideal polyvalent vehicle for nanomachine fabrication, pathogen detection, and the delivery of therapeutics. This report describes novel approaches for the fabrication...
Adhesion of micro and nanoparticles onto cardiovascular walls is a critical process in applications such as targeted drug delivery, biomedical imaging, and cancer treatment. This paper intends to develop an understanding of the dynamic interaction between particle and vessel wall through computational modeling. The ligand-receptor binding dynamics is coupled with immersed finite element method to...
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