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Intracellular protein phosphatase activity has been identified in the yeast Yarrowia lipolytica. This activity was maximal early in its exponential growth phase, and it was enhanced by Pi-deficiency of the culture medium. On a Pi-deficient medium, the major protein phosphatase was purified. This enzyme was dissociated with 80% ethanol treatment, its activity was slightly increased (30%) with heparine...
Antisense oligonucleotides are currently used for the specific control of the expression of a selected gene. Their putative targets are located in the cytoplasm (messenger RNA) or the nucleus (pre-messenger RNA or DNA). This approach is conditioned by the presence of the antisense molecule inside the cell at sufficient concentrations and in the appropriate compartments. We propose in this paper...
The chemical diversification of DNA precursors was undertaken in Escherichia coli by expressing the human gene for deoxycytidine kinase, and supplying such recombinant strains with nucleoside analogues bearing an altered base or sugar. Arabinocytidine and dideoxycytidine thus became highly toxic to E. coli in the sub-millimolar range. Deoxynucleosides bearing isoadenine (2-aminopurine) and isoguanine...
CEM lymphoid cells expressing high levels of HIV-1 nef protein displayed a significant decrease in basal phosphatidyl-inositol 3-kinase (PI3-kinase) activity associated with immunoprecipitates with anti-p85 regulatory subunit. In contrast, chronically infected U937 monocytic cells displayed a significant increase in basal PI3-kinase activity in cells infected with HIV-1 nef - compared to...
In plants, the transfer of the sulfur atom between cysteine and homocysteine, the direct precursor of methionine, is ensured by two chloroplastic enzymes, cystathionine γ-synthase and cystathionine β-lyase. These proteins have been purified to homogeneity from spinach chloroplasts and their biochemical properties determined. Cystathionine γ-synthase and cystathionine β-lyase are tetramers and are...
Alkaline phosphatase activity (ALP) (EC: 3.1.3.1) presents a nycthemeral variation in both Microcystis aeruginosa (cyanobacterium) and Synedra capitata (diatom) species. Nevertheless, a comparative study reveals differences between the enzymatic behaviour of these two species. ALP is 33 times higher in cyanobacteria than in diatoms under similar experimental conditions. Microcystis aeruginosa presents...
Two isoforms of a cadmium-binding protein (Cd-BP 14a and Cd-BP 14b) were isolated from the terrestrial oligochaete annelid, Allolobophora caliginosa. The complete amino acid sequence of the major isoform Cd-BP 14a (molecular mass: 13441 Da; 119 residues) and the amino-terminal sequence (57 residues) of Cd-BP 14b were determined. The sequence of Cd-BP 14a is highly similar to that of myohemerythrins...
It has been shown that micro-injections of angiotensin II in the Organum cavum pre-lamina terminalis (OCPLT) of the rat brain trigger water intake. Our anatomical study shows that the anterior, vertical extension of this Organum corresponds to the anterior inter-hemispheric fissure, and its posterior, horizontal extension to the Cavum septi pellucidi (CSP). Contrary to classical descriptions, the...
A new compound, carbamoyl-pyridoxal 5 -phosphate (C-PLP), was synthetized by condensation of pyridoxal 5 -phosphate (PLP) with KCNO. It may be obtained under certain physiological conditions of pH, temperature and concentration of reagents. Formation and degradation of C-PLP are readily reversible chemical reactions, not involving enzymes, at least in rat tissues. However, different considerations...
Comparative studies of highly repeated DNA from different species of Indriidae (Primates, Strepsirhini) allowed confirmation of the specific status of Avahi occidentalis, A. laniger and Propithecus tattersalli. The comparison of their band patterns revealed the existence of specific and common bands from which a cladogram of the family is inferred. This cladogram shows that Avahi clade is the sister-group...
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