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Isolation of cells from heterogeneous biological samples is critical in both basic biological research and clinical diagnostics. Affinity-based methods, such as those that recognise cells by binding antibodies to cell membrane biomarkers, can be used to achieve specific cell isolation. Microfluidic techniques have been employed to achieve more efficient and effective cell isolation. By employing aptamers...
In this paper, we present two designs for developing a biocompatible affinity MEMS glucose sensor. Based on these designs, two devices were fabricated and characterized. These devices, consisting of a micro-cantilever or membrane situated inside microchambers, are driven by a remote magnetic field and separated from the sensing environment by a semi-permeable membrane. A change in viscosity induced...
We present a novel microfluidic sensor that is capable of detecting trace cocaine concentrations and is fully regenerable at modest temperatures. The sensor exploits affinity aptamers (synthetic DNA/RNA oligonucleotides), labeled with a fluorophore and immobilized onto polymer microbeads as a highly sensitive cocaine receptor medium. The device demonstrates the capability of detecting native cocaine...
Here, we present a "smart" microfluidic system for highly selective extraction of trace amounts of analytes using ribonucleic acid (RNA) aptamers. A micro-chamber consisting of streptavidin coated polystyrene beads was fabricated on a glass substrate using standard MEMS fabrication techniques. The beads were mobilized into the reaction chamber by an auxiliary channel and trapped using two...
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