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Altered metabolism is a hallmark of cancer that opens new therapeutic possibilities. 2‐deoxyglucose (2‐DG) is a non‐metabolizable glucose analog tested in clinical trials and is frequently used in experimental settings to mimic glucose starvation. However, in the present study, conducted in a rhabdomyosarcoma cell line, we find that 2‐DG induces classical nuclear apoptotic morphology and caspase‐dependent...