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The conserved C-terminal FATC domain of the kinase ‘target of rapamycin’ is important for its regulation and was suggested to contain a peripheral membrane anchor. Here, we present the characterization of the interactions of the yeast TOR1 FATC domain (2438–2470=y1fatc) and 15 mutants with membrane mimetic micelles, bicelles, and small unilamellar vesicles (SUVs) by NMR and CD spectroscopy. Replacement...
Previous work has demonstrated improved diagnostic performance of highly trained breast radiologists when provided with B-mode plus elastography images over B-mode images alone. In those studies we have observed that elasticity imaging can be difficult to perform if there is substantial motion of tissue out of the image plane. So we are extending our methods to 3D/4D elasticity imaging with 2D arrays...
We have begun testing the ability to perform modulus reconstructions (absolute elastic parameter) imaging of in vivo breast tissues. The reconstructions are based on high quality 2D displacement estimates from strain imaging. Piecewise linear (secant) modulus reconstructions demonstrate the changes in elasticity image contrast seen in strain images but, unlike the strain images, the contrast in the...
Elasticity (mechanical strain) imaging is under rapid development as a tool to improve the specificity of breast ultrasound imaging. Elasticity imaging exploits the fact that benign and malignant breast disease cause inherently different tissue stiffness. We performed a retrospective observer study to determine if elasticity imaging can improve radiologists risk assessment for breast masses over risk...
We are objectively investigating lesion contrast with ultrasound strain imaging of in vivo solid breast lesions. Tissue deformation is performed during real-time strain imaging. A modified block matching algorithm is used to estimate the displacement between frames of radiofrequency (RF) echo data and the gradient of the displacements are computed to form strain images. Displacement estimates from...
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