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A maltogenic amylase from fenugreek (Trigonella foenum graecum) seeds was extracted and then purified to homogeneity. Optimization extraction was carried out using Box Benkhen Design (BBD). An overall purification of about 239-folds with an activity yield of 47% was achieved. The pure enzyme has an apparent molecular mass of 68kDa, as determined by SDS–PAGE analysis. Its maximal activity was in the...