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Tissue-specific distribution of γδ TCRs with limited TCR diversity is a common phenomenon in species with a low percentage of γδ T cells like humans and mice. We set out to investigate whether this is also the case in cattle (Bos taurus), a species with high percentages of γδ T cells. Using a method that was independent of variable (V) segment-specific primers, we generated 65 unique TCR δ chain sequences...