Molecular Immunology

article

Rheumatoid-factor-reactive sites on CH ₃ established by overlapping 7-mer peptide epitope analysis

Carol Peterson, Christine C. Malone, Ralph C. Williams

Molecular Immunology > 1995 > 32 > 1 > 57-75

Polyclonal IgM rheumatoid factors (RF) from ten patients with rheumatoid arthritis and six monoclonal IgM RF were isolated from monomeric IgG affinity columns and studied for their reactivity with the entire CH ₃ domain of IgG synthesized as overlapping 7-mers using a pin-ELISA assay. All ten polyclonal IgM RF showed similar profiles of reactivity which included peptides with solvent accessible...

article

The complete cDNA and deduced amino acid sequence of equine IgE

P. Navarro, D. P. Barbis, D. Antczak, J. E. Butler

Molecular Immunology > 1995 > 32 > 1 > 1-8

The cDNA from a transcript encoding the complete heavy chain of the equine immunoglobulin IgE has been cloned and sequenced. A fragment of the equine epsilon gene was amplified from cDNA using PCR and this fragment was then used to probe a horse cDNA library prepared from peripheral blood lymphocytes. A recombinant clone containing the cDNA encoding the complete horse epsilon chain and its associated...

article

Polymorphism of the V _α 15 T-Cell receptor subfamily

Robert J. Pogulis, Nasri E. Jacir, Larry R. Pease

Molecular Immunology > 1995 > 32 > 1 > 93-95

article

Intramolecular immunodominance and intermolecular selection of H2 ^d -restricted peptides define the same immunodominant region of the measles virus fusion protein

Claude P. Muller, Robert Bünder, Helmut Mayser, Susanne Ammon, more

Molecular Immunology > 1995 > 32 > 1 > 37-47

The generation of a sustained antibody response requires the participation of MHC class II-restricted T helper cells. We have identified class II-restricted sequences by immunizing BALB/c (H-2 ^d ) mice with 108 overlapping synthetic pentadecapeptides covering the whole sequence of the measles virus fusion protein (MV-F). Several strong T cell epitopes were found including a major cluster...

article

Covalently-bound human C4b dimers consisting of C4b isotype show higher hemolytic activity than those of C4A in the C3-bypass complement pathway

Takahisa Masaki, Misako Matsumoto, Tomoko Hara, Isao Nakanishi, more

Molecular Immunology > 1995 > 32 > 1 > 21-26

The ability to form a covalent dimer of human C4b was investigated with purified isotypes C4A and C4B, and antibody-sensitized liposomes supplemented with C1. In this system, no C4A or C4B formed a complex with the antibody or C1. Whereas both C4A and C4B isotypes formed dimers to a similar extent, C4B formed an ester-linked dimer and C4A an amide-linked dimer. Both of these dimers served as a...

article

Molecular characterization of a human V _λ _V _I _I _I germline gene

Lan-Yang Ch'ang, Maria Schell, Carol Ringelberg, Deborah T. Weiss, more

Molecular Immunology > 1995 > 32 > 1 > 49-55

Human λ light chains of the recently recognized variable region (V _L ) subgroup V _λ _V _I _I _I can be distinguished from proteins of other V _λ gene families on the basis of distinctive chemical and serologic properties and by their preferential association with certain types of autoantibodies, i.e. rheumatoid factors (RFs). We now report that we have cloned from...

article

Variable region light chain genes encoding human antibodies to HIV-1

Denis David, Moncef Zouali

Molecular Immunology > 1995 > 32 > 1 > 77-88

In previous work, it was found that the heavy chain variable gene (V _H ) repertoire of human antibodies to HIV is markedly skewed and that the gp120 molecule is a ligand for V _H 3 gene products. Here, we have analysed the light chain (L-chain) variable region genes (V _L ) expressed by a panel of human monoclonal antibodies derived from an immunized volunteer, an AIDS patient...

article

Immunoanalysis of human insulin using monoclonal antibodies reveals antigenicity of evolutionarily conserved residues

Bernard Pau, Sophie Allauzen, Sophie Joly, Claude Granier, more

Molecular Immunology > 1995 > 32 > 1 > 27-36

Seven anti-human insulin monoclonal antibodies (mAb) were produced according to an efficient immunization protocol elaborated in our laboratory. Their affinity constants for the binding to the insulin molecule ranged from 5.0 10 ⁸ M ^- ¹ to 1.0 10 ¹ ⁰ M ^- ¹ when insulin was in solution and from 6.0 10 ⁶ M ^- ¹ to 2.5 10 ...

article

Electron spin resonance study of the metal binding site of glucose specific peripheral blood lymphocyte lectin

Rajeev Kayestha, Krishnan Hajela, Sudhakar Bharti

Molecular Immunology > 1995 > 32 > 1 > 97-100

The electron spin resonance (ESR) spectrum of Mn ² ⁺ is highly responsive to changes in coordination symmetry. We have thus used ESR spectroscopy to study Mn ² ⁺ bound to goat peripheral blood lymphocyte lectin to delineate the nature of the metal binding site of the lectin. Our results suggest the presence of two metal binding sites on goat peripheral blood lymphocyte lectin,...

article

Chimeric interleukin 2 receptor α chain antibody derivatives with fused μ and γ chains permit improved recruitment of effector functions

Michaela Schäffner, Christine François, Brigitte Kaluza, Yannick Jacques, more

Molecular Immunology > 1995 > 32 > 1 > 9-20

In order to investigate the feasibility of shuffling effector functions of monoclonal antibodies, we constructed chimeric antibodies with fused heavy chains. The derivatives studied are based on a monoclonal antibody directed against the α chain of the human I12-R. Derivatives studied were the IgG1 and IgM isotypes; IgMΔ, lacking the ability of multimerization due to a deletion; IgMcγ 1 and IgG1cμ,...

article

Cloning and characterization of murine CD6

Gena Whitney, Michael Bowen, Michael Neubauer, Alejandro Aruffo

Molecular Immunology > 1995 > 32 > 1 > 89-92

The lymphocyte antigen CD6 has been extensively studied in humans, but has not been reported in mouse. Here we describe the isolation and characterization of a cDNA clone encoding the murine homologue of the human lymphocyte antigen CD6. Comparison of the predicted amino acid sequence of human and murine CD6 shows that the cytoplasmic domain of murine CD6 is 160 residues longer than that of the...

article

VH shuffling can be used to convert an Fv fragment of anti-hen egg lysozyme specificity to one that recognizes a T cell receptor Vα

E. Sally Ward

Molecular Immunology > 1995 > 32 > 2 > 147-156

This study describes the isolation and characterization of Fv fragments that recognize a T cell receptor Vα (Vα1934.4). A VH gene repertoire from an immunized mouse was recombined with the anti-hen egg lysozyme (HEL) VκD1.3 gene as single chain (sc)Fvs, and an Fv with reasonable affinity for binding to Vα1934.4 isolated. The Fv (VH14/VκD1.3) does not bind to HEL, indicating that the heavy chain...

article

Evidence for an early heavy chain intermediate in the assembly of H-2Db class I MHC molecules

Linda S. Cauley

Molecular Immunology > 1995 > 32 > 2 > 137-146

Several recently proposed models for the in vivo biogenesis of class I MHC molecules focus on the retention of empty dimers as a postulated intermediate in the assembly of the complete complexes. The data presented in this study support a slightly different model of class I biogenesis, which includes a precursor population of H-2Db heavy chains (HCs) that is retained in the ER of murine cells prior...

article

Inhibition of T cell activation with a humanized anti-β1 integrin chain mAb

Marie-Alix Poul, Michel Ticchioni, Alain Bernard, Marie-Paule Lefranc

Molecular Immunology > 1995 > 32 > 2 > 101-116

The murine anti-CD29 mAb K20 (Mu-K20) is known to bind to the β1 chain of the human integrins and to inhibit activation and proliferation of T cells, implying an important potential for in vivo immunosuppression. However, use of K20 as an immunosuppressant drug would be impaired by the immunogenicity of mouse mAbs in man. We have therefore engineered K20 into (1) a mouse/human chimeric mAb (Ch-K20)...

article

An early post-mutational selection event directs expansion of autoreactive B cells in murine lupus

Joseph P. Portanova, Geralyn Creadon, Xianghua Zhang, Diana S. Smith, more

Molecular Immunology > 1995 > 32 > 2 > 117-135

We report evidence for a strong selection event directing the outgrowth of autoreactive B cells in spontaneous murine lupus. The event occurred shortly following the induction of the somatic hypermutation process. This conclusion is derived from extensive sequence analyses of V _H and V _L loci expressed by hybridomas representing two large histone-specific clones (lineages) from an...

article

Cyclosporin-sensitive expression of cytokine mRNA in mouse macrophages responding to bacteria

Ulf Svensson, Elisabet Holst, Roger Sundler

Molecular Immunology > 1995 > 32 > 2 > 157-165

Characteristics of the cytokine response in resident mouse macrophages to certain Gram-positive and Gram-negative bacteria have been investigated by monitoring the expression of mRNA encoding interleukin-1α and -β (IL-1α/β) and tumor necrosis factor-α (TNF-α). Expression of these cytokine mRNAs occurred within 30-60 min. Both the flavonoid quercetin and phloretin inhibited the expression of IL-1α/β...

article

Hydrophobic, hydrophilic and other interactions in epitope-paratope binding

C. J. Van Oss

Molecular Immunology > 1995 > 32 > 3 > 199-211

Macroscopic, non-covalent, aspecific interactions between hydrophilic biopolymers, particles and cells in aqueous media tend to be repulsive; they are caused by Lifshitz-van der Waals (LW), Lewis acid-base (AB) and electrostatic (EL) forces. Microscopic scale specific interactions, e.g. between epitopes and paratopes, are also non-covalent and caused by attractive LW, AB and EL forces, which locally...

article

Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli

Hervé Le Calvez, Jacques Fieschi, J. Michael Green, Nathalie Marchesi, more

Molecular Immunology > 1995 > 32 > 3 > 185-198

Two monoclonal antibodies (mAbs), 5A4 and 6D6, directed against cortisol, have been obtained; 6D6 is used in an assay kit for cortisol. The antibodies also recognize other, structurally related steroids present in the sample assayed. To improve the specificity of the assay, we aimed to minimize the recognition of non-cortisol steroids by the two anti-cortisol mAbs. Our strategy consisted in constructing...

article

Gene rearrangement patterning and Dnase-I hypersensitive sites within the T-cell receptor Jα locus

Vibhuti P. Dave, Julia L. Hurwitz

Molecular Immunology > 1995 > 32 > 3 > 229-232

For several years, the relationship between αβ and γδ T-cell progenitors has been a topic of debate. Some argue that a subset of T-cell progenitors is pre-committed to the αβ lineage and is thus programmed to rearrange α, but not δ genes. It is further argued that the deletion of the δ locus by a unique rearrangement, δrec-ΨJα, may be the critical forerunner to V-Jα joins in αβ committed cells...

article

Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum

Gernot Achatz, Hannes Oberkofler, Erich Lechenauer, Birgit Simon, more

Molecular Immunology > 1995 > 32 > 3 > 213-227

The two moulds, Alternaria alternata and Cladosporium herbarum, are recognized as major causes of fungal allergies. Cloning, sequencing and heterologous expression of the allergens of the two moulds is a necessary step in understanding fungal allergy and in the development of new and improved methods of diagnosis and therapy. The seven new mould allergens presented here represent four new allergen...

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Molecular Immunology

Rheumatoid-factor-reactive sites on CH ₃ established by overlapping 7-mer peptide epitope analysis

The complete cDNA and deduced amino acid sequence of equine IgE

Polymorphism of the V _α 15 T-Cell receptor subfamily

Intramolecular immunodominance and intermolecular selection of H2 ^d -restricted peptides define the same immunodominant region of the measles virus fusion protein

Covalently-bound human C4b dimers consisting of C4b isotype show higher hemolytic activity than those of C4A in the C3-bypass complement pathway

Molecular characterization of a human V _λ _V _I _I _I germline gene

Variable region light chain genes encoding human antibodies to HIV-1

Immunoanalysis of human insulin using monoclonal antibodies reveals antigenicity of evolutionarily conserved residues

Electron spin resonance study of the metal binding site of glucose specific peripheral blood lymphocyte lectin

Chimeric interleukin 2 receptor α chain antibody derivatives with fused μ and γ chains permit improved recruitment of effector functions

Cloning and characterization of murine CD6

VH shuffling can be used to convert an Fv fragment of anti-hen egg lysozyme specificity to one that recognizes a T cell receptor Vα

Evidence for an early heavy chain intermediate in the assembly of H-2Db class I MHC molecules

Inhibition of T cell activation with a humanized anti-β1 integrin chain mAb

An early post-mutational selection event directs expansion of autoreactive B cells in murine lupus

Cyclosporin-sensitive expression of cytokine mRNA in mouse macrophages responding to bacteria

Hydrophobic, hydrophilic and other interactions in epitope-paratope binding

Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli

Gene rearrangement patterning and Dnase-I hypersensitive sites within the T-cell receptor Jα locus

Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum

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