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Loose interaction between the glycolytic enzymes glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was visualized in living CHO‐K1 cells by fluorescence resonance energy transfer (FRET), using time‐domain fluorescence lifetime imaging microscopy. FRET between active tetrameric subunits of GAPDH linked to cerulean or citrine was observed, and this FRET signal was significantly...