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The clusters of regularly interspaced short palindromic repeats (CRISPR) and the Cas (CRISPR‐associated) proteins form an adaptive immune system in bacteria and archaea that evolved as an RNA‐guided interference mechanism to target and degrade foreign genetic elements. In the so‐called type IIIA CRISPR‐Cas systems, Cas proteins from the Csm family form a complex of RNPs that are involved in surveillance...
The clusters of regularly interspaced short palindromic repeats (CRISPR)–CRISPR‐associated proteins (Cas) system consists of an intriguing machinery of proteins that confer bacteria and archaea with immunity against phages and plasmids via an RNA‐guided interference mechanism. Here, the cloning, recombinant expression in Escherichia coli BL21 (DE3), purification, crystallization and preliminary X‐ray...
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