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New therapeutic approaches for repairing an injured or degenerating nervous system have accelerated the development of methods to generate populations of neurons derived from various stem cell sources efficiently. Many of these methods require the generation of neurospheres. Here a simple technique is described for creating an array of adherent mouse embryonic stem cell (mESC)‐derived neurospheres...
Embryonic stem cells provide a potential promising therapeutic approach for tissue regeneration. With the advent of microfluidic technology we can more closely recapitulate features of the native tissue environment. Here we use a contemporary compartmentalized device to culture mouse embryonic stem cells (mESCs) and differentiate them with a defined neuronal medium into process-extending “neurons”...
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