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Western blot is a principal technique for the detection of specific proteins in various biology disciplines. The antibody incubation is an imperative step in western blot. Antibody incubation by mild shaking on a rocker is generally used to facilitate mixing of antibodies. However, mild shaking is an inefficient process to remove antibody depletion layer on blotting membrane and requires hours of...
DOI: 10.1002/elps.201800195
The cover picture shows the rotational cyclic draining and replenishing (CDR) procedure demonstrated with the traditional irrigation wheel, ‘Rehat’ used in rural Pakistan. The rotational CDR incubation of western blot membrane in antibody solution allows fast and efficient antibody binding to target proteins. We designed a cylindrical chamber for rotational CDR antibody...
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