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In arbuscular mycorrhizal (AM) symbiosis the plasma membrane of both, roots and fungi, is regarded to be the main contact zone between the two symbionts. Therefore plant proteins for regulating symbiotic interactions are expected to be located at the plasma membrane of roots. It has been shown that plasma membrane-bound nitrate reductase (PM-NR) and nitrite: NO reductase (NI-NOR) are tightly bound...
The effect of NO radicals on the adhesion and spreading of unilamellar DMPC and DOPC liposomes on mercury electrodes were studied by chronoamperometry. NO was either generated in situ from NOC-18 or supplied as an admixture of nitrogen gas. These measurements reveal details of the kinetics of the adhesion and spreading process and provide access to information on the membrane fluidity of lipid bilayer...
Plant plasma membrane (pm) vesicles from mycorrhizal tobacco (Nicotiana tabacum cv. Samsun) roots were isolated with negligible fungal contamination by the aqueous two-phase partitioning technique as proven by fatty acid analysis. Palmitvaccenic acid became apparent as an appropriate indicator for fungal membranes in root pm preparations. The pm vesicles had a low specific activity of the vanadate-sensitive...
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