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In order to preserve sufficient fluorescence intensity and improve the quality of fluorescence images in optical projection tomography (OPT) imaging, a feasible acquisition solution is to temporally formalize the fluorescence and bright-field imaging procedure as two consecutive phases. To be specific, fluorescence images are acquired first, in a full axial-view revolution, followed by the bright-field...
In biological studies, Saccharomyces cerevisiae yeast cells are used to study the behaviour of proteins. This is a time consuming and not completely objective process. Hence, image analysis platforms are developed to address these problems and to offer analysis per cell as well. The segmentation algorithms implemented in such platforms can segment the healthy cells, along with artefacts such as debris...
The yeast Saccharomyces cerevisiae is used to understand gene expression and genetic networks. To study the levels and localization of individual proteins, tagging with fluorescent proteins like GFP is widely used. An important issue is quantification of the fluorescent signal in microscopic images. In addition to microscopy, Image Analysis has developed as an established complement allowing routine...
Cell migration is essential in a number of processes, including wound healing, angiogenesis and cancer metastasis. Especially, invasion of cancer cells in the surrounding tissue is a crucial step that requires increased cell motility. Cell migration is a well-orchestrated process that involves the continuous formation and disassembly of matrix adhesions. Those structural anchor points interact with...
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