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Circular and ovoidal objects such as cells and supramolecular complexes are very common in bio-imaging. In order to do measurements on such objects, the accurate detection of these objects from microscope images is essential. Therefore our objective is finding accurate and reliable methods to extract the contour delineating the object. In earlier work, we have shown to be successful in achieving this...
In biological studies, Saccharomyces cerevisiae yeast cells are used to study the behaviour of proteins. This is a time consuming and not completely objective process. Hence, image analysis platforms are developed to address these problems and to offer analysis per cell as well. The segmentation algorithms implemented in such platforms can segment the healthy cells, along with artefacts such as debris...
The yeast Saccharomyces cerevisiae is used to understand gene expression and genetic networks. To study the levels and localization of individual proteins, tagging with fluorescent proteins like GFP is widely used. An important issue is quantification of the fluorescent signal in microscopic images. In addition to microscopy, Image Analysis has developed as an established complement allowing routine...
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