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This paper presents an electrokinetically controlled microfluidic chip that allows investigation of binding interactions between nucleic acids and target cells. The microchip consists of two microchambers for capture of cells using an integrated microweir structure and collection of DNA strands, respectively. The chambers are connected by a microchannel filled with agarose gel. In the chip, nucleic...
This paper presents electrokinetically based microfluidic integration of isolation and amplification of target-binding nucleic acids. A microfluidic device is used that consists of two microchambers for nucleic acid isolation and amplification connected by a microchannel filled with agarose gel. In the device, target-binding DNA strands are isolated and amplified on surfaces while gel-based electrophoresis...
This paper presents a microchip that effectively isolates and enriches target-binding single-stranded DNA (ssDNA) in a randomized DNA mixture using solid-phase extraction (SPE) and electrophoresis. The microchip consists of isolation and enrichment microchambers that are connected by a microchannel partially filled with agarose gel. Single-stranded DNA oligomers in the randomized mixture are captured...
This paper presents a microfluidic device which uses bead-based polymerase chain reaction (PCR) to amplify and detect genomic DNA of Bordetella Pertussis. PCR is a biochemical amplification process in which template DNA is duplicated by repeated thermal cycling and enzymatic amplification. The device uses an integrated resistive heater and temperature sensor beneath a (poly) dimethylsiloxane microfluidic...
We present a microfluidic biosensor suitable for selective detection of analytes with integrated analyte preconcentration, isocratic elution and mass spectrometric detection. The device uses an aptamer (i.e., oligonucleotide that binds specifically to an analyte via affinity coupling) immobilized on microbeads to achieve highly selective analyte capture and concentration. Here, we demonstrate specific...
Ni nanowires (NWs) are driven in water using a rotating magnetic field. The NWs show various locomotion performances under different boundary conditions. Pure rotation occurs when the two ends of the NWs are homogenously constrained. However, if the boundary conditions are different at the two ends of the NWs, tumbling motion, i.e., rotation plus translation, will be generated, which leads to the...
We present an innovative microfluidic device that accomplishes integrated, all-aqueous realization of specific extraction, concentration, and coupling to mass spectrometric detection of biomolecular analytes. The device uses an aptamer (i.e., oligonucleotide that binds specifically to an analyte via affinity coupling) immobilized on microbeads to achieve highly selective analyte capture and concentration...
We present a device for selective extraction, concentration and release of metabolic molecules using ribonucleic acid (RNA) aptamers. The device consists of microfabricated PDMS channels on a glass substrate including a micro-chamber packed with an aptamer stationary phase. Features include: extraction and preconcentration of trace amounts of adenosine monophosphate (model analyte); sufficiently low...
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