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A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental...
Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis...
This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated...
Periprosthetic joint infection (PJI) is a devastating complication after arthroplasty. Two-stage reimplantation procedure is the most common practice to prevent the reoccurrence of PJI. However, it should be assisted by a rapid, accurate and comprehensive bacteria detection tool. In this study, a new integrated microfluidic system capable of performing sample pre-treatment, a nanogold detection to...
Periprosthetic joint infection (PJI) is difficult to treat and the incidence is between 1% and 2% in primary arthroplasties. Implant-associated infections usually arise via either primary infections from bacterial invasion at the time of implant surgery or secondary infections from hematogenous sources. The two-stage re-implantation protocol that consists of extensive debridement at the first stage...
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