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Introduction: Although the multilineage potential of human processed lipoaspirate has previously been demonstrated, few studies exist utilizing mouse adipose-derived mesenchymal cells (ADMSCs) in vitro or in vivo. Here, we develop a method for osteogenic differentiation of ADMSCs in vitro and utilize them to repair calvarial defects in vivo.Methods: ADMSCs were harvested from 3 week old female FVB...
Introduction: Data indicates that TGF-betas may play an important role in craniosynostosis. In a murine organ culture model, we previously demonstrated that blocking TGF-beta signaling leads to pathologic suture patency. In this study, we investigated both the osteogenic properties of TGF-beta1 and determined whether adenoviral overexpression of TGF-beta1 in a normally patent sagittal (SAG) suture...
Introduction: Guided tissue regeneration (GTR) utilizes barrier materials to create environments that favor tissue regeneration. In the current study, we applied the principle of GTR to our rat mandibular distraction osteogenesis (DO) model in an attempt to enhance bone regeneration.Methods: Osteoconductive Gore Resolut XT membranes (a co-polymer of polylactic acid and polyglycolic acid) were used...
Introduction: Cleft palate is a common disorder that occurs when there is failure of the palatal shelves to fuse during normal development. The genes mediating normal palatal fusion are largely unknown.Methods: We harvested mRNA and performed microarray (~40,000 element) analysis of mice palatal shelves at timepoints before, during, and after palatal fusion (E13.5, E14.5, and E15.5). IHC was performed...
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