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Supercritical fluid chromatographic (SFC) separations of a-tocopheryl acetate, α-, ß-, y- and -tocopherol have been achieved on a Spherisorb S3 ODS2 column in about 15 minutes. Separation efficiency of this method has been optimised with respect to the effect of pressure, percent of methanol in the mobile phase, temperature and flow rate, resulting in theoretical plate numbers (N) varying from 63000...
Supercritical fluid extraction (SFE) both with CO2 and CO2/hexane has been developed as a method of analysis for lipids in cruciferous oilseed, rapeseed products and lupin. The SFE procedure used for quantitative determination of oil was compared to traditional Soxhlet oil extraction. The quantitative values obtained with SFE were equal to those obtained with the Soxhlet procedure with respect to...
Dietary fibres (DF) have been isolated from rapeseed and peas, separated into different fractions and investigated for their content of associated non-carbohydrate compounds, especially proteins by UV-spectroscopy, nitrogen determination, isoelectric focusing, and rocket Immunoelectrophoresis. The level of protein varied according to the plant origin of DF and among the different DF fractions (pectins,...
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