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Using optical tweezers for trapping and infection of influenza virus influenza virus binds selectively G1 phase cell. This result encouraged us to dissolve the difference between G1- and S/G2/M-phage cells. At the beginning, we tried to analyze the sialic acid content of both G1-and S/G2/M-phase cells. Because it had been shown that influenza virus attached to the sialic acid on the cell membrane...
In this paper, we developed transfection of a single fluorescent nanosensor into a cell nucleus using step-wise fusion of liposome for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using Layer-by-Layer formation. The sensor is manipulated by optical tweezers and transfected into the cell or cell nucleus by membrane fusion...
In this paper, we developed injection of a single fluorescent nanosensor into a specific cell by using membrane fusion of liposome and optical tweezers for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using spontaneous transfer of phospholipid-coated sensor through an oil/water interface. The sensor is manipulated by optical...
Optical tweezers which enables to trap micron to nanometer sized objects by radiation pressure force is utilized for manipulation of particles under a microscope and for measurement of forces between biomolecules. Weak force of optical tweezers causes some limitations such as particle adhesion or steric barrier like lipid membrane in a cell prevent further movement of objects. For biomedical applications...
We developed an active virus filter (AVF) that was able to virus enrichment for single virus manipulation by using the insulator based dielectrophoresis (iDEP). The design of 3D constricted flow channel enabled the microfluidic chip to produce iDEP force. We utilized maskless photolithography to achieve the precise 3D gray-scale exposure for constricted flow channel. When we applied sinusoidal wave...
In this paper, we proposed a long-lifetime temperature measurement using color shift of the fluorescence from the quantum dots. The cell investigation tool encapsulates the quantum dots and can adhere to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We used image processing of fluorescent image of the tools to acquire the color information from the fluorescent...
Influenza virus attaches on the sialic acid on cellular membrane through it's HA protein and enters cytoplasm by receptor-mediated endocytosis, then move from endocytic vesicles to early endosomes and late endosome where the influenza viruses fuse with the endosomal membrane to release viral genome followed to transport the genome to nuclei where virus replicate their genome. Previously we found that...
We propose in situ formation of gel microbeads made of a thermoreversible hydrogel for indirect laser micromanipulation of microorganisms, DNA and viruses. Irradiation, using a 1064 nm laser, of an aqueous solution mixed with poly-(N-isopropylacrylamide) through a high magnification lens resulted in the formation of a gel microbead at the laser focus due to heating. The gel microbead was trapped by...
Influenza virus RNA polymerase is composed of three subunits, PB1, PB2 and PA, which is involved in both transcription and replication of viral genome RNA, negative strand RNA. Influenza virus gene product RNA dependent RNA polymerase synthesizes two different RNAs, one is mRNA and another is cRNA which become a template for virus genome RNA synthesis. To synthesize two different RNAs the RNA polymerase...
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