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We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it...
The single cell analysis has attracted much attention to reveal the detailed and localized biological information, which is not obtained by the conventional group cell analysis due to the statistical assay, on individual cells. To analyze the detailed and localized properties of single cells, local environmental control technique, which is the technique to change and sense the local environment around...
The goal of this article is to make a pattern of human induced pluripotent stem cells (hiPSCs) on a Polydimethylsiloxane (PDMS) surface. Here, we report that pattering of hiPSCs can be achieved by patterned plasma treatment followed by coating with a two protein mixture. The result is useful for future applications of micro devices in human cell culture.
In this research, we demonstrated a microfluidic chip to pretreat the samples for viral genome assay. The microfluidic chip has the following three functions; (1) Virus purification and enrichment, (2) Viral RNA extraction, and (3) Capture of the targeted virus genome. Hydroxyapatite chromatography, Boom method, and PNA (Peptide Nucleic Acid) were used for the above three functions, respectively....
We present an innovative gel actuator made of photo-processible thermoresponsible gel. (Fig. 1) We evaluated integratability of the gel actuator by experimentally and theoretically and confirmed that it is possible to integrate within 100 μm. We performed demonstration of integrated valves fabricated on five branched microchannel.
We proposed a 3D assembly technique of small-diameter blood vessels using a PLCL (poly (L-lactide-co-ε-caprolactone)) scaffold. The technique uses a residual stress of PLCL scaffolds to fabricate a multilayer structured tubular tissue, and gives a tissue mechanical property which blood vessels originally have. In the future, we try to test the circulatory culture system in order to investigate whether...
We proposed hybrid nanorobot that can be manipulated by optical tweezers and has hybrid structure integrating functional nanomaterials. We employed femtosecond laser exposure for fabrication of the nanorobot having 3D structure with nanometric size and integrating functional nanomaterials. We succeeded in fabricating nanorobot with carbon nanotubes (CNTs) that has a high photothermal efficiency. We...
The CTCs (Circulating Tumor Cells) isolation method enables to sort very rare single CTC from the whole blood. We utilized the technique of convective self-assembly, which is a technique for depositing cells onto the microfluidic channel, to isolate CTCs. Through the convective self-assembly processes, the cells including CTCs and blood cells are deposited in the channels of microfluidic chip. After...
By use of optical tweezers, micron to nanometer sized objects can be manipulated. This method has possibilities to realize surgery of a cell and investigation of mechanical property of a cell. In this article a novel scheme to enhance instantaneous radiation pressure force by use of a pulse laser and a measurement scheme are presented.
Microsphere-based therapy is used to allow drug release to treat the critical site through the choice and formulation of variations drug-polymer combinations. They are also used to control the release of insoluble drug by the biodegradability of polymer. Chitosan is one of the candidates for the polymer matrix because their biodegradability and biocompatibility. Recently, we prepared and characterized...
We proposed a 3D assembly technique and a circulatory culture system. The technique gives multilayer-structured tubular tissues for blood vessel models. By the circulatory culture system, this study was aimed to determine whether the tissue-engineered tubular models maintain the normally mechanical property as the development and function. In this work, we were able to fabricate tubular tissues from...
This study performed multiple micro-contact printing of extra-cellular matrix with a fine alignment system for the position and the posture of polydimethylsiloxane (PDMS) stamp. The position and the posture were adjusted with a 4-axis automated stage with a resolution of 1 μm or 0.0025 deg. The adjustment was achived by monitoring the surface shape of PDMS stamp measured by using a laser displacement...
Gas flows through a micro-channel were experimentally studied by using PSCC (Pressure-Sensitive Channel Chip). PSCC is a micro-channel fabricated by PDMS containing a pressure-sensitive dye, and then it works as both a micro-channel and PSP (Pressure-Sensitive Paint). The micro-channel (PSCC) with 200 × 91 μm cross section and 2.0 mm long was fabricated. The pressure distribution with the inlet and...
This paper reports a novel on-chip cellular force measurement method of single cells using Direct-Outer-Drive mechanism (D-O-D mechanism). This D-O-D mechanism enables high speed (1 kHz), high power (1 kN) and high accurate manipulation (nanometer-order) of Robochip (Robot-integrated microfluidic chip) and mechanical property measurement of single cells. We succeeded in mechanical property measurement...
High resolution imaging is an important technique for micro-nano scale objects to obtain the structural information. Micro computed tomography (micro-CT) is one of the techniques to obtain internal information without physical damage for sample. We investigate the micro-CT imaging for the Caenorhabditis elegans (C. elegans) in this paper. This is the one of the smallest animal used as a model organism,...
The present study was designed to investigate whether octacalcium phosphate (OCP) in alginate beads promote mouse osteoblastic differentiation of mouse bone marrow stromal cells. The result suggested that OCP/alginate beads could be a better composite used in bone regenerative therapy.
We have developed a poly(dimethylsiloxane) (PDMS)-made microfluidic long-term cell culture platform having two cell culture wells with piezoelectric substrate bottom on which shear horizontal surface acoustic wave (SH-SAW) propagates. The SH-SAW propagates on the cells could be used to assay cell-cell and/or cell-surface interaction. The platform also enables optical observation of the cells and acquisition...
This paper reports fabrication of retinal microvessels model for evaluation of microcannulation. Because microcannulation as a new treatment for retinal vein occlusion is difficult surgery operation, development and evaluation of microsurgical robot systems to assistant vitreoretinal surgery is needed. Evaluation of microcannulation needs a retinal microvessels model without individual difference...
We proposed the new method to assemble 3D cell structures using hysteresis character of thermoresponsive polyer solution. In this paper, we conducted cell culture inside thermoresponsive polymer solution to check whether cells can grow inside the gel. A ultra-low attachment flask was used to culture cells for preventing cell attachment on the surface and check the effect when cells were embedded inside...
We developed selective transfection of single fluorescence sensor into a specific cell by optically controlled cell immobilization and membrane fusion of lipid capsule for intracellular measurement. The single fluorescent sensor was encapsulated in the lipid capsule using novel packaging based on a multi-layering method. The liposomes with number-controlled lipid bulyer contain 1,3,3-Trimethylindolino-6'-nitrobenzopyrylospiran...
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