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Next generation DNA sequencing is used to determine the HLA‐A, ‐B, ‐C, ‐DRB1, and ‐DQB1 assignments of 1472 unrelated volunteers for the unrelated donor registry in Argentina. The analysis characterized all HLA exons and introns for class I alleles; at least exons 2, 3 for HLA‐DRB1; and exons 2 to 6 for HLA‐DQB1. Of the distinct alleles present, there are 330 class I and 98 class II. The majority...
Traditional DNA‐based typing focuses primarily on interrogating the exons of human leukocyte antigen (HLA) genes that form the antigen recognition domain (ARD). The relevance of mismatching donor and recipient for HLA variation outside the ARD on hematopoietic stem cell transplantation (HSCT) outcomes is unknown. This study was designed to evaluate the frequency of variation outside the ARD in 10...
We have updated the catalogue of common and well‐documented (CWD) human leukocyte antigen (HLA) alleles to reflect current understanding of the prevalence of specific allele sequences. The original CWD catalogue designated 721 alleles at the HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3/4/5, ‐DQA1, ‐DQB1, and ‐DPB1 loci in IMGT (IMmunoGeneTics)/HLA Database release 2.15.0 as being CWD. The updated CWD catalogue designates...
In order to reduce the time required to identify a match for unrelated donor hematopoietic stem cell transplantation, a one‐step DNA sequencing strategy was employed at the time of recruitment. The impact of this strategy on human leukocyte antigen (HLA) typing resolution and the effect of current registry requirements on resolution and coding of assignments were evaluated. Sanger‐based DNA sequencing...
SummaryThe goal of the immunogenomic data analysis working group (IDAWG) is to facilitate the consistent analysis of HLA and KIR data, and the sharing of those data among the immunogenomic and larger genomic communities. However, the data management approaches currently applied by immunogenomic researchers are not widely discussed or reported in the literature, and the effect of different approaches...
HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1 assignments were obtained for 374 pairs of African American mothers and their umbilical cord blood units (CBU) by DNA sequencing. An algorithm developed by the National Marrow Donor Program was used to assign 1122 haplotypes by segregation. Seventy percent of the haplotypes carried assignments at all five loci. In the remainder, alleles at various loci, most often DQB1...
Seventy‐two novel human leukocyte antigen (HLA) class I and class II alleles are described from volunteers for the ‘Be The Match Registry®’: 17 HLA‐A alleles, 12 HLA‐C alleles, 31 HLA‐B alleles and 12 HLA‐DRB1 alleles. Forty‐six (∼64%) of the 72 novel alleles are single‐nucleotide substitution variants when compared with their most homologous allele. Five of these single‐nucleotide variants are silent...
Twenty‐one novel human leukocyte antigen (HLA) class I and class II alleles are described: seven HLA‐A alleles, five HLA‐C alleles, five HLA‐B alleles and four HLA‐DRB1 alleles. Seventeen (∼81%) of the 21 novel alleles are single nucleotide substitution variants when compared with their most homologous allele. Nine of these single nucleotide variants cause an amino acid substitution, while eight are...
Ninety‐six novel human leukocyte antigen (HLA) class I and class II alleles are described from volunteers for the ‘Be The Match Registry®’: 15 HLA‐A alleles, 11 HLA‐C alleles, 36 HLA‐B alleles and 34 HLA‐DRB1 alleles. Sixty‐eight (∼71%) of the 96 novel alleles are single nucleotide substitution variants when compared with their most homologous allele. Twenty‐three of these single nucleotide variants...
Seventy‐eight novel human leukocyte antigen (HLA) class I and class II alleles are described: 19 HLA‐A alleles, 30 HLA‐C alleles, 21 HLA‐B alleles, 7 HLA‐DRB1 alleles and 1 HLA‐DPB1 allele. Eight (∼10%) of the novel alleles were found in more than one individual and may be more common in the population. Sixty‐two (∼80%) of the 78 novel alleles are single nucleotide substitution variants when compared...
Sixty‐five novel human leukocyte antigen (HLA) alleles are described from volunteer donors of the ‘Be The Match Registry®’: 29 HLA‐A alleles, 24 HLA‐B alleles, and 12 HLA‐DRB1 alleles. Eight (∼12%) of the novel alleles were found in more than one individual and may be more common in the population. Forty (∼62%) of the 65 novel alleles are single nucleotide substitution variants when compared with...
KIR3DL1 and KIR3DS1 allele frequencies were determined by DNA sequencing of the complete coding regions from 100 random unrelated African Americans. Alleles 3DL1*01501 (29 individuals) and 3DL1*01502 (23 individuals) were most frequently observed in addition to 21 other known alleles and 7 new alleles: KIR3DL1*01503, *03102, *064, *065, *066, *067, and *068. Two of the new alleles (KIR3DL1*064, *065)...
KIR2DL1 alleles were identified by DNA sequencing of the coding region from amplified genomic DNA from 100 random African Americans. The majority of individuals (97%) carried a KIR2DL1 locus. Allele KIR2DL1*00302 was found in 68% of individuals but KIR2DL1*00401, *002, *00303, *006, and *007 were also frequent. Eleven new alleles were described: KIR2DL1*00403, *01101, *01102, *012, *013N, *014, *015, *016, *017, *018...
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