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Background: Treatment of malignant tumors of the pelvis, especially in the periacetabular region continues to present one of the most challenging problems in oncological and orthopedic surgery. Even now, hindquarter amputation is still the only choice for some patients, but hindquarter amputation can cause much trouble in sitting, standing and in fitting the exoprosthesis of patients after amputation,...
This study was to examine whether there is evidence for vasculogenic mimicry in osteosarcoma. In vivo and in vitro studies were conducted. Studies using hematoxylin and eosin (HE) stain and CD31 immunohistochemical stain to observe the micro vascular channels in human osteosarcoma specimens were performed. Osteosarcoma cell lines (U-20S) were also tested in vitro for their ability to form tubular...
Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast...
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