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We proposed sensor-integrated retina model with similar Young's modulus to human retina for training of ophthalmologist. Mimic of mechanical properties of human retina is important issue for surgical simulator. Moreover, integration of sensor into surgical model is useful for evaluation of surgeon skill. First, we fabricate eye ball model having retina and scleral with similar Young's modulus to human...
We proposed on-chip spatiotemporal sensing for monitoring interactions between cells and environment using fluorescence multi-microsensors. The microfluidic chip integrating fluorescence multi-microsensor is used to construct the harmonious environment like in vivo to reconstruct high-quality organs ex vivo. Fluorescence sensing is suitable for spatiotemporal sensing with high spatial resolution because...
Effective manipulation and rapid injection of nanosensor with high cell viability have great significance to biological and biomedical applications such as drug delivery and cells biology. This study presents manipulation of a magnetic nanosensor, immobilization onto cell surface using zeta potential control and injection into cell cytoplasm by local laser heating. The nanosensor is stained by a temperature...
We proposed on-chip spatiotemporal fluorescence sensing for monitoring interactions between cells and environment. As a demonstration, Spatiotemporal variation of Calcium ion was measured and during transformation of octacalcium phosphate (OCP) to hydroxyl apatite (HA) in the microfluidic chip.
This paper presents the 3D vessel model having a temperature Indicator for evaluating thermal damage during surgery. The materials of model are polydimethylsiloxane (PDMS) and thermo chromic dye which indicates color change according to temperature increase. The vessel model is fabricated by dip coating on 3D wax model and lost wax method. However, acetone for removing wax prevents the temperature...
Effective manipulation and rapid injection of nanosensor with high cell viability have great significance to biological and biomedical applications such as drug delivery and cells biology. This study presents manipulation of a magnetic nanosensor with a temperature indicator and immobilization onto cell surface using glass nanoprobe by Coulomb's force. The nanosensor is stained by Rhodamine B as the...
Rapid and effective injection of functional nanobeads into a specific living cell with high cell viability is one of the most important issues for single cell analysis. This paper reports a novel method of injecting single magnetic nanobeads into a living Madin-Darby canine kidney (MDCK) cell using a continuous-wave near infrared laser. Experimental results demonstrated the successful injection of...
We developed unconstraint measurement of vital information using near-infrared light sensor for casual sensing of pulse wave information. This sensor is composed of infrared LED and photodiode for measuring the cyclic change of hemoglobin concentration inside blood vessel. This sensor could measure the pulse wave without constraint of human body. Measured data represented near value to the measured...
We proposed novel hybrid gel microtool made of a polystyrene microbead coated with thermo-sensitive gel, Poly (N-isopropylacrylamide) (PNIPAAm). In this study, we confirmed that cell adhesion properties of the microtool could be changed using laser heating during trapping by optical tweezers. Furthermore, we demonstrated single cell transportation by adhering the cell to the microtool with optical...
This paper reports temperature indicator for surgical simulator and temperature calculation method using image processing for visualization of temperature increase and distribution in the arterial biological model. The indicator consists of polydimethylsiloxane (PDMS) and thermochromic pigment presenting non-reversible color change according to temperature. The indicator has transparency to observe...
We proposed novel optically-driven microtool which can control cell adhesion on its surface by laser heating. The microtool is made of polystyrene microbead coated with thermo-responsive hydrogel, Poly (N-isopropylacrylamide) (PNIPAAm). Target cells adhere to the surface of the microtool at the temperature higher than Lower Critical Solution Temperature (LCST) and do not adhere at the temperature...
Optical heating of metallic nanoparticles is of great significance to biological and biomedical applications, such as gene/molecule delivery and intracellular microenvironment measurement. In this study, we propose to employ optical heating of metallic nanoparticles to achieve fast injection of single nanoscale sensor into living cells. The nanoscale sensor consists of polystyrene bead, which is embedded...
Evaluation of oocyte and embryo has become important for many biomedicai applications. We developed measurement of oxygen consumption rate of individual oocyte using fluorescence oxygen sensor having stripe-shape on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The...
We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS)....
We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it...
We developed selective transfection of single fluorescence sensor into a specific cell by optically controlled cell immobilization and membrane fusion of lipid capsule for intracellular measurement. The single fluorescent sensor was encapsulated in the lipid capsule using novel packaging based on a multi-layering method. The liposomes with number-controlled lipid bulyer contain 1,3,3-Trimethylindolino-6'-nitrobenzopyrylospiran...
In this paper, we developed transfection of a single fluorescent nanosensor into a cell nucleus using step-wise fusion of liposome for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using Layer-by-Layer formation. The sensor is manipulated by optical tweezers and transfected into the cell or cell nucleus by membrane fusion...
We developed fabrication and manipulation of hybrid nanorobots by 3D nano exposure and optical tweezers. Hybrid nanorobot is composed of photoresist and silicon nanowire. The robot is fabricated by femtosecond laser exposure and is connected to the glass substrate by a micropillar. The processing resolutions of the femtosecond laser exposure are 270 nm (line width) and 600 nm (thickness), respectively...
In this paper, we developed injection of a single fluorescent nanosensor into a specific cell by using membrane fusion of liposome and optical tweezers for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using spontaneous transfer of phospholipid-coated sensor through an oil/water interface. The sensor is manipulated by optical...
Carbon nanotube (CNT) is one of the most promising materials for various applications. There are a lot of studies on mechanical and thermal properties of a single CNT in vacuum condition. However, thermal conductivity of the single CNT in liquid are not well investigated. In this paper, we proposed evaluation of thermal conductivity of a single CNT using fluorescence temperature sensor and local laser...
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