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To construct rapidly a full–length cDNA library from nanogram amounts total RNA of Giardia lamblia (G. lamblia) trophozoites stocked in RNA stabilization reagent. Total RNA of Giardia was extracted using Trizol reagent. A full–length cDNA library of G. lamblia trophozoites was constructed by a long-distance PCR (LD-PCR) method. The recombinant rate and the coverage rate of full–length clones of...
Considering the protection of existing tunnel and significant changes of the earth pressure below, it is very difficult to select reasonable chamber pressure in undercrossing existing subway construction. Under the different buried depth of existing tunnel, characteristics of earth pressure distribution below are studied with the numerical simulation. It is shown that the lateral range and depth of...
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