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We describe in details the materials and methods used to construct, calibrate and evaluate tissue phantoms and light sources; we describe the method for acquiring the data and present quantitative results for light intensity reconstruction using the reproducible phantom's optical properties as a priori information. We consider a tissue phantom imaging with optical properties which match those for...
The electron-multiplying charge-coupled device (EMCCD) offers improved quantum efficiencies (40 to 95%) over a broader range of wavelengths (400 to 900 nm) and a higher intrinsic resolution (<100 mum using photon counting) when compared to photomultiplier tubes. The electron gain achieved in the multiplication register of an EMCCD effectively reduces the readout noise to less than 1 electron/pixel,...
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