Search results for: Hidenori Akutsu

Items from 1 to 20 out of 37 results

article

Familial Pseudohypoparathyroidism Type IB Associated with an SVA Retrotransposon Insertion in the GNAS Locus

Sayaka Kawashima, Akiko Yuno, Shinichiro Sano, Akie Nakamura, more

Journal of Bone and Mineral Research > 37 > 10 > 1850 - 1859

Loss of methylation (LOM) at GNAS‐A/B:TSS‐differentially methylated regions (DMRs) in the GNAS locus is observed in pseudohypoparathyroidism type 1B (PHP1B). Many PHP1B cases are sporadic, but autosomal dominant‐PHP1B has a deletion involving NESP55 expressed from the maternal allele or STX16 located upstream of the GNAS locus on the maternal allele. We report the possible first familial PHP1B cases...

article

Low‐density cell culture enhances hepatic function through tight junction formation in HepG2 cells

Rieko Tanaka‐Yachi, Kazuko Aizawa, Kie shimizu, Hidenori Akutsu, more

Biology of the Cell > 114 > 9 > 225 - 236

Background Information An in vitro evaluation system using cultured hepatocytes is the most useful method in preclinical research, such as drug metabolism and toxicity test. Human hepatocytes should be used in an in vitro evaluation system because the expression of drug‐metabolizing enzymes varies among animal species. HepG2 cells, a liver cancer‐derived cell line, are widely used as a human hepatocyte...

article

Imprinted X‐chromosome inactivation impacts primitive endoderm differentiation in mouse blastocysts

Atsushi Fukuda, Nami Motosugi, Mikiko Ando, Minoru Kimura, more

FEBS Letters > 594 > 5 > 913 - 923

Epigenetic and transcriptome alterations are essential for lineage specification, represented by imprinted X‐chromosome inactivation (iXCI) in female mouse preimplantation embryos. However, how various factors affect transcriptome states and lineage commitment remains unclear. We found that in vitro culture duration strongly influences transcriptional variation compared to iXCI loss. Single‐cell analysis...

article

DNA hypermethylation enhanced telomerase reverse transcriptase expression in human-induced pluripotent stem cells

Ken Takasawa, Yoshikazu Arai, Mayu Yamazaki-Inoue, Masashi Toyoda, more

Human Cell > 2018 > 31 > 1 > 78-86

During reprogramming into human induced pluripotent stem cells (iPSCs), several stem cell marker genes are induced, such as OCT-4, NANOG, SALL4, and TERT. OCT-4, NANOG, and SALL4 gene expression can be regulated by DNA methylation. Their promoters become hypomethylated in iPSCs during reprogramming, leading to their induced expression. However, epigenetic regulation of the TERT gene remains unclear...

article

Characteristics of the placental differentiation process analyzed using human induced pluripotent stem cells

Junya Kojima, Atushi Fukuda, Hayato Taira, Shigehiro Hayashi, more

Placenta > 2017 > 57 > C > 295

article

Fetal Therapy Model of Myelomeningocele with Three-Dimensional Skin Using Amniotic Fluid Cell-Derived Induced Pluripotent Stem Cells

Kazuhiro Kajiwara, Tomohiro Tanemoto, Seiji Wada, Jurii Karibe, more

Stem Cell Reports > 2017 > 8 > 6 > 1701-1713

Myelomeningocele (MMC) is a congenital disease without genetic abnormalities. Neurological symptoms are irreversibly impaired after birth, and no effective treatment has been reported to date. Only surgical repairs have been reported so far. In this study, we performed antenatal treatment of MMC with an artificial skin using induced pluripotent stem cells (iPSCs) generated from a patient with Down...

article

In vivo maturation of human embryonic stem cell-derived teratoma over time

Hidenori Akutsu, Michiyo Nasu, Shojiroh Morinaga, Teiichi Motoyama, more

Regenerative Therapy > 2016 > 5 > Complete > 31-39

Transformation of human embryonic stem cells (hESC) is of interest to scientists who use them as a raw material for cell-processed therapeutic products. However, the WHO and ICH guidelines provide only study design advice and general principles for tumorigenicity tests. In this study, we performed in vivo tumorigenicity tests (teratoma formation) and genome-wide sequencing analysis of undifferentiated...

article

Human induced pluripotent stem cells (iPS cell) differentiate into trophoblast lineage

Junya Kojima, Hidenori Akutsu, Naoaki Kuji, Keiichi Isaka

Placenta > 2016 > 46 > C > 121

article

Human induced pluripotent stem cells (iPS cell) differentiate to trophoblast lineage in xeno free cluture

Junya Kojima, Hidenori Akutsu, Hirotaka Nishi, Naoaki Kuji, more

Placenta > 2016 > 45 > C > 127

article

Human cytomegalovirus downregulates SLITRK6 expression through IE2

Huanan Liao, Haruna Sato, Ryosuke Chiba, Tomoko Kawai, more

Journal of NeuroVirology > 2017 > 23 > 1 > 79-86

Congenital human cytomegalovirus (HCMV) infection causes sensorineural hearing loss (SNHL) and other neurological disorders, although the neuropathogenesis of HCMV infection is not well understood. Here, we show that the expression of SLITRK6, one of causative genes for hereditary SNHL, was robustly downregulated by HCMV infection in cultured neural cells. We also show that HCMV-encoded immediate-early...

article

Induced pluripotent stem cells (iPS cell) differentiates to trophoblast

Junya Kojima, Hidenori Akutsu, Hirotaka Nishi, Naoaki Kuji, more

Placenta > 2015 > 36 > 10 > A5

article

Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells

Hidenori Akutsu, Masakazu Machida, Seiichi Kanzaki, Tohru Sugawara, more

Regenerative Therapy > 2015 > 1 > Complete > 18-29

The potential applications of human embryonic stem cells (hESCs) in regenerative medicine and developmental research have made stem cell biology one of the most fascinating and rapidly expanding fields of biomedicine. The first clinical trial of hESCs in humans has begun, and the field of stem cell therapy has just entered a new era. Here, we report seven hESC lines (SEES-1, -2, -3, -4, -5, -6, and...

article

Ethical acceptability of research on human-animal chimeric embryos: summary of opinions by the Japanese Expert Panel on Bioethics

Hiroshi Mizuno, Hidenori Akutsu, Kazuto Kato

Life Sciences, Society and Policy > 2015 > 11 > 1 > 1-7

Human-animal chimeric embryos are embryos obtained by introducing human cells into a non-human animal embryo. It is envisaged that the application of human-animal chimeric embryos may make possible many useful research projects including producing three-dimensional human organs in animals and verification of the pluripotency of human ES cells or iPS cells in vivo. The use of human-animal chimeric...

article

A melanocyte–melanoma precursor niche in sweat glands of volar skin

Natsuko Okamoto, Takahiro Aoto, Hisashi Uhara, Satoshi Yamazaki, more

Pigment Cell & Melanoma Research > 27 > 6 > 1039 - 1050

Determination of the niche for early‐stage cancer remains a challenging issue. Melanoma is an aggressive cancer of the melanocyte lineage. Early melanoma cells are often found in the epidermis around sweat ducts of human volar skin, and the skin pigmentation pattern is an early diagnostic sign of acral melanoma. However, the niche for melanoma precursors has not been determined yet. Here, we report...

article

Differentiation of Human Induced Pluripotent Stem Cells into Functional Enterocyte-like Cells Using a Simple Method

Takahiro Iwao, Masashi Toyota, Yoshitaka Miyagawa, Hajime Okita, more

Drug Metabolism and Pharmacokinetics > 2014 > 29 > 1 > 44-51

Human induced pluripotent stem (iPS) cells were differentiated into the endoderm using activin A and were then treated with fibroblast growth factor 2 (FGF2) for differentiation into intestinal stem cell-like cells. These immature cells were then differentiated into enterocyte-like cells using epidermal growth factor (EGF) in 2% fetal bovine serum (FBS). At the early stage of differentiation, mRNA...

article

An Efficient Method for Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-like Cells Retaining Drug Metabolizing Activity

Yuki Kondo, Takahiro Iwao, Katsunori Nakamura, Takamitsu Sasaki, more

Drug Metabolism and Pharmacokinetics > 2014 > 29 > 3 > 237-243

The use of human induced pluripotent stem (iPS) cells would be of great value for a variety of applications involving drug development studies. Several reports have been published on the differentiation of human iPS cells into hepatocyte-like cells; however, the cells were insufficient for application in drug metabolism studies. In this study, we aimed to establish effective methods for differentiation...

article

Investigation of telomere length dynamics in induced pluripotent stem cells using quantitative fluorescence in situ hybridization

Masanori Terai, Naotaka Izumiyama-Shimomura, Junko Aida, Naoshi Ishikawa, more

Tissue and Cell > 2013 > 45 > 6 > 407-413

Here we attempted to clarify telomere metabolism in parental cells and their derived clonal human induced pluripotent stem cells (iPSCs) at different passages using quantitative fluorescence in situ hybridization (Q-FISH). Our methodology involved estimation of the individual telomere lengths of chromosomal arms in individual cells within each clone in relation to telomere fluorescence units (TFUs)...

article

Establishment and directed differentiation of induced pluripotent stem cells from glycogen storage disease type Ib patient

Daisuke Satoh, Tohru Maeda, Tetsuya Ito, Yoko Nakajima, more

Genes to Cells > 18 > 12 > 1053 - 1069

Glycogen storage disease type Ib (GSDIb) is caused by a deficiency in the glucose‐6‐phosphate transporter (G6PT), which leads to neutrophil dysfunction. However, the underlying causes of these dysfunctions and their relationship with glucose homeostasis are unclear. Induced pluripotent stem cells (iPSCs) hold a great promise for advances in developmental biology, cell‐based therapy and modeling of...

article

Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer

Miwako Nishio, Takeshi Yoneshiro, Masako Nakahara, Shinnosuke Suzuki, more

Cell Metabolism > 2012 > 16 > 5 > 684-685

article

MicroRNA‐199a‐3p, microRNA‐193b, and microRNA‐320c are correlated to aging and regulate human cartilage metabolism

Taku Ukai, Masato Sato, Hidenori Akutsu, Akihiro Umezawa, more

Journal of Orthopaedic Research® > 30 > 12 > 1915 - 1922

MicroRNAs (miRNAs) are small RNAs of ∼22 base pairs that regulate gene expression. We harvested cartilage tissue from patients with polydactylism, anterior cruciate ligament injury, and osteoarthritis undergoing total knee arthroplasty and used microarrays to identify miRNAs whose expression is upregulated or downregulated with age. The results were assessed by real‐time PCR and MTT assay in a mimic...

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INFONA - science communication portal

Search results for: Hidenori Akutsu

Familial Pseudohypoparathyroidism Type IB Associated with an SVA Retrotransposon Insertion in the GNAS Locus

Low‐density cell culture enhances hepatic function through tight junction formation in HepG2 cells

Imprinted X‐chromosome inactivation impacts primitive endoderm differentiation in mouse blastocysts

DNA hypermethylation enhanced telomerase reverse transcriptase expression in human-induced pluripotent stem cells

Characteristics of the placental differentiation process analyzed using human induced pluripotent stem cells

Fetal Therapy Model of Myelomeningocele with Three-Dimensional Skin Using Amniotic Fluid Cell-Derived Induced Pluripotent Stem Cells

In vivo maturation of human embryonic stem cell-derived teratoma over time

Human induced pluripotent stem cells (iPS cell) differentiate into trophoblast lineage

Human induced pluripotent stem cells (iPS cell) differentiate to trophoblast lineage in xeno free cluture

Human cytomegalovirus downregulates SLITRK6 expression through IE2

Induced pluripotent stem cells (iPS cell) differentiates to trophoblast

Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells

Ethical acceptability of research on human-animal chimeric embryos: summary of opinions by the Japanese Expert Panel on Bioethics

A melanocyte–melanoma precursor niche in sweat glands of volar skin

Differentiation of Human Induced Pluripotent Stem Cells into Functional Enterocyte-like Cells Using a Simple Method

An Efficient Method for Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-like Cells Retaining Drug Metabolizing Activity

Investigation of telomere length dynamics in induced pluripotent stem cells using quantitative fluorescence in situ hybridization

Establishment and directed differentiation of induced pluripotent stem cells from glycogen storage disease type Ib patient

Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer

MicroRNA‐199a‐3p, microRNA‐193b, and microRNA‐320c are correlated to aging and regulate human cartilage metabolism

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