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Thermoalkaliphilic Bacillus sp. JB-99 was grown in a 250 ml Erlenmeyer flask containing 50 ml medium containing (g/l) Pigeon pea waste 10; NaNO 3 , 5.0; K 2 HPO 4 , 5.0; MgSO 4 .2H 2 O, 0.2 and Na 2 CO 3 , 10.0. Incubations were carried out at 50 o C on a rotary incubator shaker for 15 h. A high level of extra cellular thermostable protease...
The thermophilic and alkaliphilic Bacillus sp. JB-99 was isolated from sugarcane molasses and was cultured in 250 ml Erlenmeyer flasks containing 50 ml of synthetic medium consisting of (g/l): citric acid; 10.0, NaNO 3 ; 10.0, K 2 HPO 4 ; 5.0, MgSO 4 .7H 2 O; 0.3, CaCl 2 .2H 2 O; 0.2, NaCl; 5.0 and Na 2 CO 3 ; 10.0 at pH 10.0...
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