Thermoalkaliphilic Bacillus sp. JB-99 was grown in a 250 ml Erlenmeyer flask containing 50 ml medium containing (g/l) Pigeon pea waste 10; NaNO 3 , 5.0; K 2 HPO 4 , 5.0; MgSO 4 .2H 2 O, 0.2 and Na 2 CO 3 , 10.0. Incubations were carried out at 50 o C on a rotary incubator shaker for 15 h. A high level of extra cellular thermostable protease activity was observed after 24 h incubation. The optimum temperature and pH for activity were 70 o C and 11, respectively, so this enzyme showed stable activity at high temperature and under alkaline conditions.