Search results for: Koichi Kuwano

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SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice

Yutaka Kida, Takeshi Yamamoto, Koichi Kuwano

Microbiology and Immunology > 64 > 4 > 280 - 295

Mucin is a glycoprotein that is the primary component of the mucus overlaying the epithelial tissues. Because mucin functions as a first line of the innate immune system, Pseudomonas aeruginosa appears to require interaction with mucin to establish infection in the host. However, the interactions between P. aeruginosa and mucin have been poorly understood. In this study, using in vivo expression technology...

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Mycoplasma pneumoniae protects infected epithelial cells from hydrogen peroxide‐induced cell detachment

Takeshi Yamamoto, Yutaka Kida, Koichi Kuwano

Cellular Microbiology > 21 > 6 > n/a - n/a

Epithelial cell shedding is a defence mechanism against infectious microbes that use these cells as an infection foothold and that eliminate microbes from infection foci by removing infected cells. Mycoplasma pneumoniae, a causative agent of respiratory infections, is known to adhere to and colonise the surface of ciliated airway epithelial cells; it produces a large amount of hydrogen peroxide, indicating...

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Mpn491, a secreted nuclease of Mycoplasma pneumoniae, plays a critical role in evading killing by neutrophil extracellular traps

Takeshi Yamamoto, Yutaka Kida, Yuichi Sakamoto, Koichi Kuwano

Cellular Microbiology > 19 > 3 > n/a - n/a

Neutrophils play an important role in antimicrobial defense as the first line of innate immune system. Recently, the release of neutrophil extracellular traps (NETs) has been identified as a killing mechanism of neutrophils against invading microbes. Mycoplasma pneumoniae, a causative agent of respiratory infection, has been shown to be resistant to in vitro killing by neutrophils, suggesting that...

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Effects of Ureaplasma parvum lipoprotein multiple-banded antigen on pregnancy outcome in mice

Kaoru Uchida, Kumiko Nakahira, Kazuya Mimura, Takashi Shimizu, more

Journal of Reproductive Immunology > 2013 > 100 > 2 > 118-127

Ureaplasma spp. are members of the family Mycoplasmataceae and have been considered to be associated with chorioamnionitis and preterm delivery. However, it is unclear whether Ureaplasma spp. have virulence factors related to these manifestations. The purpose of the present study was to determine whether the immunogenic protein multiple-banded antigen (MBA) from Ureaplasma parvum is a virulence factor...

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EprS, an autotransporter protein of Pseudomonas aeruginosa, possessing serine protease activity induces inflammatory responses through protease‐activated receptors

Yutaka Kida, Junichi Taira, Takeshi Yamamoto, Yuichiro Higashimoto, more

Cellular Microbiology > 15 > 7 > 1168 - 1181

PA3535 (EprS), an autotransporter (AT) protein of Pseudomonas aeruginosa, is predicted to contain a serine protease motif. The eprS encodes a 104.5 kDa protein with a 30‐amino‐acid‐long signal peptide, a 51.2 kDa amino‐terminal secreted passenger domain and a 50.1 kDa carboxyl‐terminal outer membrane channel formed translocator. Although the majority of AT proteins have been reported to be virulence...

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Impaired capsular polysaccharide is relevant to enhanced biofilm formation and lower virulence in Streptococcus pneumoniae

Liang Qin, Yutaka Kida, Yoshihiro Imamura, Koichi Kuwano, more

Journal of Infection and Chemotherapy > 2013 > 19 > 2 > 261-271

Streptococcus pneumoniae has been reported to form biofilms. Many different surface molecules, including capsular polysaccharide (CPS), may play a fundamental role in pneumococcal biofilm development. We designed a CPS mutant, TIGR4cps4D⁻, from the TIGR4 strain and detected enhanced biofilm formation. The pathogenic diversities of the mutant were also investigated with the in vitro expression levels...

article

Impaired capsular polysaccharide is relevant to enhanced biofilm formation and lower virulence in Streptococcus pneumoniae

Liang Qin, Yoshihiro Imamura, Hiroshi Watanabe, Yutaka Kida, more

Journal of Infection and Chemotherapy > 2013 > 19 > 2 > 261-271

Streptococcus pneumoniae has been reported to form biofilms. Many different surface molecules, including capsular polysaccharide (CPS), may play a fundamental role in pneumococcal biofilm development. We designed a CPS mutant, TIGR4cps4D ⁻ , from the TIGR4 strain and detected enhanced biofilm formation. The pathogenic diversities of the mutant were also investigated with the in vitro expression...

article

Reactive oxygen species mediate Jak2/Stat3 activation and IL-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins from Mycoplasma pneumoniae

Sang Yong Choi, Joo Weon Lim, Takashi Shimizu, Koichi Kuwano, more

Inflammation Research > 2012 > 61 > 5 > 493-501

Objective To investigate the involvement of reactive oxygen species (ROS) in the activation of Janus kinase2 (Jak2)/signal transducers and activators of transcription3 (Stat3), and IL-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins (LAMP) from Mycoplasma pneumoniae using a known antioxidant, N-acetylcysteine (NAC). Methods Pulmonary epithelial A549 cells...

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Mycoplasma pneumoniae infection induces a neutrophil‐derived antimicrobial peptide, cathelin‐related antimicrobial peptide

Kenji Tani, Takashi Shimizu, Yutaka Kida, Koichi Kuwano

Microbiology and Immunology > 55 > 8 > 582 - 588

In innate immunity, cationic antimicrobial peptides including cathelin‐related antimicrobial peptide (CRAMP) are known to play critical roles in protecting the host from infection by invasive microbes, including Gram‐positive and ‐negative bacteria. However, little is known about the interactions between CRAMP and mycoplasmas. In the present study, the antimicrobial activity of CRAMP against M. pneumoniae...

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Effects of pentamidine on QT interval and ether-a-go-go related gene expression in cynomolgus monkeys

Koichi Kuwano, Junko Matsuo, Shinya Ohkuma, Masaaki Ban, more

Journal of Pharmacological and Toxicological Methods > 2011 > 64 > 1 > e46

article

Cytoadherence‐dependent induction of inflammatory responses by Mycoplasma pneumoniae

Takashi Shimizu, Yutaka Kida, Koichi Kuwano

Immunology > 133 > 1 > 51 - 61

Pathogenesis of Mycoplasma pneumoniae infection is considered to be in part attributed to excessive immune responses. Mycoplasma pneumoniae shows strong cytoadherence to host cells and this cytoadherence is thought to be involved in the progression of pneumonia. However, the interaction between the cytoadherence and the immune responses is not known in detail. In this study, we demonstrated that the...

article

Modifications on amphiphilicity and cationicity of unnatural amino acid containing peptides for the improvement of antimicrobial activity against pathogenic bacteria

Junichi Taira, Yutaka Kida, Hiroshi Yamaguchi, Koichi Kuwano, more

Journal of Peptide Science > 16 > 11 > 607 - 612

The widespread natural sources‐derived cationic peptides have been reported to reveal bacterial killing and/or growth‐inhibiting properties. Correspondingly, a number of artificial peptides have been designed to understand antibacterial mechanism of the cationic peptides. These peptides are expected to be an alternative antibiotic against drug‐resistant pathogenic bacteria because major antimicrobial...

article

Can an individualized and comprehensive care strategy improve urinary incontinence (UI) among nursing home residents?

Yukiko Tanaka, Kumiko Nagata, Tomoe Tanaka, Koichi Kuwano, more

Archives of Gerontology and Geriatrics > 2009 > 49 > 2 > 278-283

Urinary incontinence (UI) is one of the most common and distressing conditions among nursing home residents. Although scheduled care is usually provided for them, incontinence care should be individualized regarding going to the toilet, changing diapers, and taking food and water. We have developed an individualized and comprehensive care strategy to address the problem. We conducted an intervention...

article

Evaluation of QT interval using a linear model in individual cynomolgus monkeys

Tadashi Koga, Koichi Kuwano, Go Kito, Koji Kanefuji

Journal of Pharmacological and Toxicological Methods > 2007 > 55 > 3 > 265-270

Introduction: The cynomolgus monkey, one of a number of primate species phylogenetically close to humans, is commonly used in cardiovascular research, but a method for determination of the RR interval-corrected QT interval in this species needs greater consideration. The objectives of this study were to determine a method for evaluating QT interval in cynomolgus monkeys individually, disregarding...

article

Sodium butyrate up-regulates cathelicidin gene expression via activator protein-1 and histone acetylation at the promoter region in a human lung epithelial cell line, EBC-1

Yutaka Kida, Takashi Shimizu, Koichi Kuwano

Molecular Immunology > 2006 > 43 > 12 > 1972-1981

The antimicrobial protein cathelicidin is considered to play an important role in the defense mechanisms against bacterial infection. Recent studies show that sodium butyrate induces cathelicidin gene expression in human colonic, gastric and hepatic cells. However, little is known about the precise regulatory mechanisms underlying sodium butyrate-induced cathelicidin gene expression. In this study,...

article

Antimicrobial Activity of Inducible Human β Defensin-2 Against Mycoplasma pneumoniae

Koichi Kuwano, Noriko Tanaka, Takashi Shimizu, Yutaka Kida

Current Microbiology > 2006 > 52 > 6 > 435-438

Defensins in innate immunity are known to play critical roles to protect the host from infection by invasive microbes, including Gram-positive and -negative bacteria. However, little is known about the interactions between defensins and mycoplasmas. Human β defensin (hBD)-2 and hBD-3, but not hBD-1, were found to exert strikingly antimicrobial activity against Mycoplasma pneumoniae. To elucidate the...

article

Dual antibacterial mechanisms of nisin Z against Gram-positive and Gram-negative bacteria

Koichi Kuwano, Noriko Tanaka, Takashi Shimizu, Kohei Nagatoshi, more

International Journal of Antimicrobial Agents > 2005 > 26 > 5 > 396-402

Nisin, an amphipathic antibiotic peptide, is produced by a number of strains of Lactococcus lactis subsp. lactis. It has been employed as a food preservative as it has a high antibacterial activity with a relatively low toxicity for humans. Nisin is known to exert a high antibacterial activity against Gram-positive but not Gram-negative bacteria. However, purified nisin Z was found to show an antibacterial...

article

Glycoglycerolipid from the Membranes of Acholeplasma laidlawii Binds to Human Immunodeficiency Virus-1 (HIV-1) and Accelerates Its Entry into Cells

Takashi Shimizu, Sumio Arai, Hiroto Imai, Tohru Oishi, more

Current Microbiology > 2004 > 48 > 3 > 182-188

We have reported previously that glycoglycerolipids derived from the membranes of Acholeplasma laidlawii, 3-O-[2-O-(-D-glucopyranosyl)-6-O-acyl--glucopyranosyl]-1,2-di-O-acyl-sn-glycerols (GAGDGs) bind to human cell lines. In addition, the GAGDGs were found to augment HIV-1 infection in human cell lines. Here we show that GAGDG binds to HIV-1 and facilitates the entry of HIV-1 into cells. The binding...

article

An Anergic Cytotoxic T Lymphocyte Clone Exhibits Granule Exocytosis-Mediated Cytotoxicity

Koichi Kuwano, Akira Akashi, Sumio Arai

Cellular Immunology > 1998 > 185 > 2 > 114-122

T cell receptor (TCR) occupancy in the absence of a costimulatory signal transforms T helper (Th) cells or cytotoxic T lymphocytes (CTL) into a state of anergy. The anergic T cells are unable to produce cytokines; nevertheless, they maintain their killing activity. We investigated the mechanisms through which anergic CTL causes lysis of target cells. Treatment of a CTL clone with phorbol myristate...

article

Involvement of Two Distinct Killing Mechanisms in Bystander Target Cell Lysis Induced by a Cytotoxic T Lymphocyte Clone

Koichi Kuwano, Sumio Arai

Cellular Immunology > 1996 > 169 > 2 > 288-293

Cytotoxic T lymphocyte (CTL) can lyse target cells by mainly two different mechanisms including granule exocytosis- and Fas-based cytotoxicity. In addition, CTL also lyse bystander target cells. In this study, we examined whether these two different killing mechanisms also cause bystander target cells lysis. EL-4 cells, representing bystander target cells, were incubated with CD8 ⁺ CTL clone...

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