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Changes in activity of the grain aphid peroxidase (Px) and polyphenol oxidase (PPO) towards phenolics isolated from leaves of black currant, sour cherry and walnut were examined. Slight increase in activity of peroxidase was found within insect tissues after 24 h of walnut extract treatment, whereas black currant and sour cherry strongly inhibited activity of this enzyme. Later on, the walnut extract...
Extensive studies have been carried out to identify plant proteins with insecticidal properties towards insects. The paper describes a test for screening toxicity and growth inhibition of five commercially available proteins such as: bromelain, honey bee venom, two lectins-wheat germ agglutinin (WGA), concanavalin A (Con A) and lysozyme in artificial diets on Sitobion avenae /F./. Grain aphid proved...
Polyphenoloxidase (PPO) plays an important role in plant resistance to insects, and in the detoxication of phenolic compounds taken in the nutrient components. Activity of PPO was examined in the ears of spring wheat and triticale. Extraction of enzymatic protein was carried out from acetone powder with the use of 0.05M phosphoric buffer of pH 7.4. The cultivars of spring wheat have possessed higher...
The aim of study was to determine the effect of grain aphid (Sitobion avenae /F./) on nutritive value of soluble protein isolated from ears of spring triticale cultivars. Nutritive value of protein was estimated by the limiting amino acid index (CS) calculated in according with to Mitchel and Block (1946), and by the integrated index of essential amino acids (EAA) calculated in with according to Oser...
Gallic and salicylic acids were extracted with 60% methanol from the ears of two winter triticale cultivars (Dagro and Malno). Separation of phenolic acids was carried out in HPLC column - Eurospher RP-18 with use of KNAUER Maxi-Star K-1000 pump. Mixture of methanol-0.1 M KH₂PO₄ (4.5:5.5) was as mobile phase. Absorbance of analysed phenolic compounds was measured at 230 nm. Obtained results showed...
Determination the quantitative and qualitative amino acid composition of albumins and globulins isolated from two cultivars of winter triticale was done using of ion-exchange chromatography. The fifteen protein amino acids in the composition of albumins and globulins were identified and determined. The present study proved that the smaller amounts protein amino acids in the fractions albumins and...
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