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Due to the important physiological functions of GABA, various methods used to produce it. Biotransformation by microorganism cells is a more promising method than others. Among the GABA-producing lactic acid bacteria, one strain with high producing capacity was isolated from Chinese naturally pickled vegetable. The strain is identified as Lactobacillus brevis, and named as L.brevis TCCC13007 based...
Kosteletzkya virginica is a halophyte species which could be used for production of industrial raw material such as biodiesel and for developing ecologically sound saline agriculture as well as for tideland soil improving. Using Rapid Amplification of cDNA Ends (RACE) method, a 1610 bp cDNA of stearoyl-ACP desaturase (SAD) gene containing a 1188 bp complete open reading fragment (ORF) was firstly...
We have cloned and expressed a novel earthworm fibrinolytic enzyme (EFE) of Lumbricus rubellus in Pichia pastoris. Its cDNA sequence (GenBank Accession No. DQ202401) revealed a 738 bp region containing an intact ORF that encodes a protein of 245 amino acid residues, containing a signal peptide of 7 amino acid residues and a mature peptide of 238 amino acid residues, designated as EFE F238. Its cDNA...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
The feeding habit of Hepialus may be involved in the parasitic process of Cordyceps sinensis. To study the feeding habit of Hepialus pui Zhang et al. larva in Tibet Mountain Sejila, the rDNA internal transcribed spacers (ITSs) were cloned and sequenced from 26 species vegetations that distributed in A, B, and C sample area. According to these ITS sequences, the species-specific PCR primers were designed...
The degradation dynamics of phenanthrene in soil was studied. The half-life of phenanthrene in the non-sterilized contaminated soil was only 5.1 d, much shorter than the same in the sterilized soil. The dominant strain of phenanthrene microbial degradation was isolated and identified by 16S rDNA as Alcaligenes sp.; its growth and degradative capability were highest at 25°C and pH of 7.0. Inoculation...
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. Pyrus pyrifolia is a commercially important fruit tree that exhibits GSI. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, an important local cultivar...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
Catechol-O-methyl transferase (COMT) influences dopamine concentration in the pre-frontal cortex (PFC). The G/A transition in the exon 4 of the COMT gene, which results in a valine (Val) to methionine (Met) amino acid substitution (Val158Met), can bring on different enzymatic activities. Much research has found that the Met/Met genotype associated with low enzymatic activity and may enhance cognitive...
The first sequence obtained from a Potyvirus infecting Canna plants (Canna spp.) revealed that it is a new strain of Bean yellow mosaic virus (BYMV), without noticeable recombination with other plant RNA viruses from this region being found. Using a degenerate primer for all Potyvirus, a DNA fragment of about 1.6kb size was amplified from total RNA samples extracted from natural-diseased Canna plants...
Molecular dynamics (MD) simulation and ab initio quantum mechanical calculations were employed to investigate the structure and dynamics of binding of QSY 21 nonfluorescent quencher to B-DNA decamer. Two binding motifs were observed: 1. Central xanthone ring of QSY 21 dye is oriented paralelly with the nearest DNA base pair and the complex is stabilized by stacking interaction between these two components...
Summary form only given. Cold atmospheric pressure plasmas offer a unique environment for treatments of soft materials, including bio-materials and living tissue. Single plasma devices can be as small as micro-meters allowing very precise treatments reducing damage to surrounding healthy living cells. It is essential to correlate direct plasma parameters with effects on bio-materials. There are various...
Chloroethylnitrosoureas (CENUs) were an important family of anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicated that their cytotoxicity was related to the interstrand crosslink of DNA. In this study, a method for the determination of DNA crosslink was established using real-time fluorescence quantitative PCR. The DNA crosslink ratio induced by two...
Lipase activity is common in staphylococci. Based on the blast of various known staphylococcal lipase DNA sequences, the degenerate primers were derived to amplify a 782-bp consensus lipase gene sequence of Staphylococcus hominis GIMT1.079, which encoded a deduced polypeptide of 260 amino acid (aa) residues. The alignment of aa sequence revealed that this 260-aa partial lipase of S. hominis shared...
To explore the feasibility of using Agilent 2100 Bioanalyzer in the detection of the three kinds of food-borne pathogens, three pairs of primers were designed according to invA gene of Salmonella ipah gene of Shigella and inv gene of Yersinia pseudotuberculosis, and then the single PCR was performed to detect specificity. Those genes were amplified by Multiplex PCR technique using specific primers,...
Abstract-An open reading frame (ORF) containing 675 nucleotides was got by sequencing the DEV gene libraries constructed by our laboratory. The ORF was identified as DEV UL45 gene by aligning with gene bank database using the software of BlastN and ORF Finder. Specific primers were designed and PCR products containing this ORF were cloned into the vector of PMD18-T. Dot blot confirmed the UL45 gene...
The JZ-curve, a new graphical expression of the gene sequence, is introduced. This representation not only avoids the limitations associated with crossing and overlapping, but also retains the biological information of gene sequences. At the same time, we constructed a new characteristic matrix, named J/J matrix. Simultaneously, the J/J characteristic matrix based on JZ-curve can describe the chemical...
Duck enteritis virus (DEV) is an alphaherpesvirinae that causes an acute, contagious, and fatal disease. In this study, the DEV-ULS3 gene from Key Laboratory of Animal Disease and Human Health of Sichuan Province was cloned and sequenced. Nucleotide sequence analysis revealed a 1032 bp open reading frame (ORF) theoretically encoding a 343-amino acid polypeptide was homologous to UL53 gene of alphaherpesvirinaes...
cDNA containing the 3' part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3' end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to 100% and 97.9% to 100% respectively...
Sequencing-by-synthesis (SBS) promises to provide a simple, effective DNA sequencing method. However, SBS creates fluorescence in the test materials and removal of fluorescence is required in order to continue reading the DNA sequence. This ability to quench the fluorescence has a wide application in many areas of research. We used the fluorescence quencher (DABCYL) to solve this problem. DABCYL absorbs...
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